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dc.contributor.author
Tron, Adriana
dc.contributor.author
Bertoncini, Carlos Walter
dc.contributor.author
Palena, Claudia
dc.contributor.author
Chan, Raquel Lia
dc.contributor.author
Gonzalez, Daniel Hector
dc.date.available
2019-10-11T12:31:02Z
dc.date.issued
2001-12
dc.identifier.citation
Tron, Adriana; Bertoncini, Carlos Walter; Palena, Claudia; Chan, Raquel Lia; Gonzalez, Daniel Hector; Combinatorial interactions of two amino acids with a single base pair define target site specificity in plant dimeric homeodomain proteins; Oxford University Press; Nucleic Acids Research; 29; 23; 12-2001; 4866-4872
dc.identifier.issn
0305-1048
dc.identifier.uri
http://hdl.handle.net/11336/85667
dc.description.abstract
Four groups of plant homeodomain proteins contain a dimerization motif closely linked to the homeodomain. We here show that two sunflower homeodomain proteins, Hahb-4 and HAHR1, which belong to the Hd-Zip I and GL2/Hd-Zip IV groups, respectively, show different binding preferences at a defined position of a pseudopalindromic DNA-binding site used as a target. HAHR1 shows a preference for the sequence 5′-CATT(A/T)AATG-3′, rather than 5′-CAAT(A/T)ATTG-3′, recognized by Hahb-4. To analyze the molecular basis of this behavior, we have constructed a set of mutants with exchanged residues (Phe→Ile and Ile→Phe) at position 47 of the homeodomain, together with chimeric proteins between HAHR1 and Hahb-4. The results obtained indicate that Phe47, but not Ile47, allows binding to 5′-CATT(A/T)AATG-3′. However, the preference for this sequence is determined, in addition, by amino acids located C-terminal to residue 53 of the HAHR1 homeodomain. A double mutant of Hahb-4 (Ile47→Phe/Ala54→Thr) shows the same binding behavior as HAHR1, suggesting that combinatorial interactions of amino acid residues at positions 47 and 54 of the homeodomain are involved in establishing the affinity and selectivity of plant dimeric homeodomain proteins with different DNA target sequences.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Oxford University Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Combinatorial interactions of two amino acids with a single base pair define target site specificity in plant dimeric homeodomain proteins
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-10T19:33:37Z
dc.identifier.eissn
1362-4962
dc.journal.volume
29
dc.journal.number
23
dc.journal.pagination
4866-4872
dc.journal.pais
Reino Unido
dc.journal.ciudad
Oxford University Press
dc.description.fil
Fil: Tron, Adriana. Universidad Nacional del Litoral; Argentina
dc.description.fil
Fil: Bertoncini, Carlos Walter. Universidad Nacional del Litoral; Argentina
dc.description.fil
Fil: Palena, Claudia. Universidad Nacional del Litoral; Argentina
dc.description.fil
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil
Fil: Gonzalez, Daniel Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.journal.title
Nucleic Acids Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/nar/29.23.4866
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