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dc.contributor.author
Bustos, Diego Martin
dc.contributor.author
Iglesias, Alberto Alvaro
dc.date.available
2019-10-11T11:58:59Z
dc.date.issued
2000-12
dc.identifier.citation
Bustos, Diego Martin; Iglesias, Alberto Alvaro; The kinetic properties of liver glucokinase and its function in glucose physiology as a model for the comprehensive study of enzymes' kinetic parameters and reversible inhibitors; John Wiley & Sons Inc; Biochemistry and Molecular Biology Education; 28; 6; 12-2000; 332-337
dc.identifier.issn
1470-8175
dc.identifier.uri
http://hdl.handle.net/11336/85662
dc.description.abstract
Glucokinase is a key enzyme in carbohydrate metabolism in mammals. Particularly relevant is the involvement of the liver enzyme for the maintenance of blood glucose concentrations. In playing this key role, the occurrence of normal kinetic parameters of liver glucokinase is critical. Modification of Km and/or Vmax results in pathological situations occurring with hypo- or hyperglycemia. Based on this, an exercise is proposed in which different mice cell lines with abnormalities in glucose utilization are analyzed to determine the metabolic alteration at a molecular level. The student must determine the cause of abnormality by processing the kinetic data. Also, the characterization of different compounds that are reversible inhibitors of the enzyme from kinetic data is required to identify a drug that can correct the pathology in each case. One major objective of the problem is to learn about the meaning of kinetic parameters of enzymes, as well as the action of different types of reversible inhibitors.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
John Wiley & Sons Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
The kinetic properties of liver glucokinase and its function in glucose physiology as a model for the comprehensive study of enzymes' kinetic parameters and reversible inhibitors
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-10T19:34:21Z
dc.journal.volume
28
dc.journal.number
6
dc.journal.pagination
332-337
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Bustos, Diego Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
dc.description.fil
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.journal.title
Biochemistry and Molecular Biology Education
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/S1470-8175(00)00027-8
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