Mostrar el registro sencillo del ítem

dc.contributor.author
Bustos, Diego Martin  
dc.contributor.author
Iglesias, Alberto Alvaro  
dc.date.available
2019-10-11T11:58:59Z  
dc.date.issued
2000-12  
dc.identifier.citation
Bustos, Diego Martin; Iglesias, Alberto Alvaro; The kinetic properties of liver glucokinase and its function in glucose physiology as a model for the comprehensive study of enzymes' kinetic parameters and reversible inhibitors; John Wiley & Sons Inc; Biochemistry and Molecular Biology Education; 28; 6; 12-2000; 332-337  
dc.identifier.issn
1470-8175  
dc.identifier.uri
http://hdl.handle.net/11336/85662  
dc.description.abstract
Glucokinase is a key enzyme in carbohydrate metabolism in mammals. Particularly relevant is the involvement of the liver enzyme for the maintenance of blood glucose concentrations. In playing this key role, the occurrence of normal kinetic parameters of liver glucokinase is critical. Modification of Km and/or Vmax results in pathological situations occurring with hypo- or hyperglycemia. Based on this, an exercise is proposed in which different mice cell lines with abnormalities in glucose utilization are analyzed to determine the metabolic alteration at a molecular level. The student must determine the cause of abnormality by processing the kinetic data. Also, the characterization of different compounds that are reversible inhibitors of the enzyme from kinetic data is required to identify a drug that can correct the pathology in each case. One major objective of the problem is to learn about the meaning of kinetic parameters of enzymes, as well as the action of different types of reversible inhibitors.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
John Wiley & Sons Inc  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject.classification
Bioquímica y Biología Molecular  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
The kinetic properties of liver glucokinase and its function in glucose physiology as a model for the comprehensive study of enzymes' kinetic parameters and reversible inhibitors  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-10-10T19:34:21Z  
dc.journal.volume
28  
dc.journal.number
6  
dc.journal.pagination
332-337  
dc.journal.pais
Estados Unidos  
dc.description.fil
Fil: Bustos, Diego Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina  
dc.description.fil
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina  
dc.journal.title
Biochemistry and Molecular Biology Education  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/S1470-8175(00)00027-8