Mostrar el registro sencillo del ítem
dc.contributor.author
Gerometta, Rosana María del Rosario
dc.contributor.author
Kumar, Sandeep
dc.contributor.author
Shah, Shaily
dc.contributor.author
Alvarez, Larry
dc.contributor.author
Candia, Oscar
dc.contributor.author
Danias, John
dc.date.available
2016-12-01T14:56:45Z
dc.date.issued
2013-12
dc.identifier.citation
Gerometta, Rosana María del Rosario; Kumar, Sandeep; Shah, Shaily; Alvarez, Larry; Candia, Oscar; et al.; Reduction of Steroid-Induced Intraocular Pressure Elevation in Sheep by Tissue Plasminogen Activator; Association For Research In Vision And Ophthalmology; Investigative Ophthalmology & Visual Science; 54; 12-2013; 7903-7909
dc.identifier.issn
0146-0404
dc.identifier.uri
http://hdl.handle.net/11336/8564
dc.description.abstract
PURPOSE. To investigate whether tissue plasminogen activator (tPA) can prevent and/or reverse steroid-induced IOP elevation in an ovine model.
METHODS. Three animal groups were subjected to bilateral steroid-induced IOP elevation using thrice daily topical ocular prednisolone administration. In the first group (N ¼ 8), one eye each of two sheep was injected intravitreally with 100 lg, 200 lg, 500 lg, or 1 mg human recombinant tPA, while contralateral eyes received vehicle. In the second group (N ¼ 2), one eye was injected intravitreally with tPA (100 lg), while contralateral eyes received vehicle containing L-arginine. In the third group (N ¼ 4), each animal received intravitreal tPA in one eye concurrently with initiation of bilateral steroid administration. IOP was monitored for the duration of the experiment. Tissues from eyes of the third group were used to determine relative gene expression.
RESULTS. In the first and second groups, IOP decreased by 9.7 (62.8) and 9.7 (61.6) mm Hg, respectively, 24 hours after tPA administration. In the third group, tPA-treated eyes did not develop IOP elevation with DIOP of 11.8 (61.3) mm Hg 8 days later. In all tPA-treated eyes, IOP remained low until the end of the study. mRNA levels in the trabecular meshwork were decreased for plasminogen activator tissue (PLAT), increased for matrix-metalloproteinase 1 (MMP-1), and stable for plasminogen activator inhibitor 1 (PAI-1), MMP-2, MMP-9, and MMP- 13 in tPA-treated eyes compared with contralateral controls. PAI-1 mRNA levels in ciliary processes also remained similar.
CONCLUSIONS. Recombinant human tPA is effective in both preventing and reversing steroidinduced IOP elevation in sheep. Tissue plasminogen activator may be useful as a therapeutic agent in steroid-induced glaucoma.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Association For Research In Vision And Ophthalmology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Intraocular Pressure
dc.subject
Tissue Plasminogen Activator
dc.subject
Trabecular Meshwork
dc.subject
Extracellular Matrix
dc.subject.classification
Oftalmología
dc.subject.classification
Medicina Clínica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Reduction of Steroid-Induced Intraocular Pressure Elevation in Sheep by Tissue Plasminogen Activator
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-11-23T18:17:12Z
dc.identifier.eissn
1552-5783
dc.journal.volume
54
dc.journal.pagination
7903-7909
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Gerometta, Rosana María del Rosario. University Of New York; Estados Unidos. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Kumar, Sandeep. State University Of New York; Estados Unidos
dc.description.fil
Fil: Shah, Shaily. State University Of New York; Estados Unidos
dc.description.fil
Fil: Alvarez, Larry. State University Of New York; Estados Unidos
dc.description.fil
Fil: Candia, Oscar. State University Of New York; Estados Unidos
dc.description.fil
Fil: Danias, John. State University Of New York; Estados Unidos
dc.journal.title
Investigative Ophthalmology & Visual Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1167/iovs.13-12801
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://iovs.arvojournals.org/article.aspx?articleid=2127542
Archivos asociados