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dc.contributor.author
Martinez, Diana
dc.contributor.author
Thompson, Carolina
dc.contributor.author
Russo, Ana Maria
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dc.contributor.author
Jacobo, Roberto
dc.contributor.author
Torioni de Echaide, Susana
dc.date.available
2016-11-30T21:57:43Z
dc.date.issued
2014-02
dc.identifier.citation
Martinez, Diana; Thompson, Carolina; Russo, Ana Maria; Jacobo, Roberto; Torioni de Echaide, Susana; Molecular Identification of Brucella Abortus Bv5 and Strain 19 in Water Buffaloes (Bubalus Bubalis) in Northeast Argentina; Int Buffalo Information Ctr; Buffallo Bulletin; 32; 2; 2-2014; 1029-1032
dc.identifier.issn
0125-6726
dc.identifier.uri
http://hdl.handle.net/11336/8544
dc.description.abstract
Buffalo (Bubalus bubalis) populations are spread across northern Argentina, and they share their habitat with bovines. Both species are susceptible to brucellosis, and they are under a National Plan of Control and Eradication. To characterize the Brucella spp. that infects buffaloes, the blood of 35 animals that tested positive to brucellosis by a complement fixation test was collected. DNA was obtained and analyzed by polymerase chain reaction using different molecular markers. The genera, species, and biovars of Brucella were established by analyzing specific regions of the genes omp31, eri, alkB, and omp2ab. Brucella spp. was identified in 15 of 35 tested buffaloes. The product of the omp31 gene identified the genera. The detection of two fragments of 297 bp and/or 1000 bp from the eri gene confirmed the presence of B. abortus S19 and wild-type B. abortus. The amplification of the alkB gene allowed the identification of B. abortus biovars characterized by fragments of 498 bp (bv1, bv2, or bv4). The simultaneous amplification of 498 bp (alkB) and 1000 bp (eri) products suggested the presence of B. abortus bv1, which is highly prevalent in the cattle of Argentina. Fragments of 827 bp and 857 bp were amplified from the omp2ab gene, and their sequences showed 100% identity with B. melitensis and B. abortus bv5 (GenBank). However, the 721 bp product (alkB) specific for B. melitensis could not be amplified. This is the first report indicating the presence of B. abortus bv5 in Latin America.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Int Buffalo Information Ctr
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Brucellosis
dc.subject
Water Buffalo
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Molecular Typification
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Brucella Abortus Bv 5
dc.subject.classification
Ciencias Veterinarias
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dc.subject.classification
Ciencias Veterinarias
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dc.subject.classification
CIENCIAS AGRÍCOLAS
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dc.title
Molecular Identification of Brucella Abortus Bv5 and Strain 19 in Water Buffaloes (Bubalus Bubalis) in Northeast Argentina
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-11-23T18:17:29Z
dc.journal.volume
32
dc.journal.number
2
dc.journal.pagination
1029-1032
dc.journal.pais
Tailandia
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dc.journal.ciudad
Phuket
dc.description.fil
Fil: Martinez, Diana. Universidad Nacional del Nordeste; Argentina
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Fil: Thompson, Carolina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela; Argentina
dc.description.fil
Fil: Russo, Ana Maria. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro de Investigaciones y Transferencia de Formosa; Argentina
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Fil: Jacobo, Roberto. Universidad Nacional del Nordeste; Argentina
dc.description.fil
Fil: Torioni de Echaide, Susana. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela; Argentina
dc.journal.title
Buffallo Bulletin
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/cabdirect.org/abstracts/20143084085.html
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://ibic.lib.ku.ac.th/e-Bulletin/IBBUSI201302173.pdf
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