Mostrar el registro sencillo del ítem

dc.contributor.author
Perez, Ana Paula  
dc.contributor.author
Altube, María Julia  
dc.contributor.author
Schilrreff, Priscila  
dc.contributor.author
Apezteguia, Gustavo Alberto  
dc.contributor.author
Santana Celes, Fabiana  
dc.contributor.author
Zacchino, Susana Alicia Stella  
dc.contributor.author
Indiani de Oliveira, Camila  
dc.contributor.author
Romero, Eder Lilia  
dc.contributor.author
Morilla, María José  
dc.date.available
2019-10-08T21:21:03Z  
dc.date.issued
2016-03  
dc.identifier.citation
Perez, Ana Paula; Altube, María Julia; Schilrreff, Priscila; Apezteguia, Gustavo Alberto; Santana Celes, Fabiana; et al.; Topical amphotericin B in ultradeformable liposomes: Formulation, skin penetration study, antifungal and antileishmanial activity in vitro; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 139; 3-2016; 190-198  
dc.identifier.issn
0927-7765  
dc.identifier.uri
http://hdl.handle.net/11336/85408  
dc.description.abstract
Aiming to improve the topical delivery of AmB to treat cutaneous fungal infections and leishmaniasis, ultradeformable liposomes containing amphotericin B (AmB-UDL) were prepared, and structural and functional characterized. The effect of different edge activators, phospholipid and AmB concentration, and phospholipid to edge activator ratio on liposomal deformability, as well as on AmB liposomal content, was tested. Liposomes having Tween 80 as edge activator resulted of maximal deformability and AmB/phospholipid ratio. These consisted of AmB-UDL of 107. ±. 8. nm diameter, 0.078-polydispersity index and -3. ±. 0.2. mV Z potential, exhibiting monomeric AmB encapsulated in the bilayer at a 75% encapsulation efficiency. After its cytotoxicity on keratinocytes (HaCaT cells) and macrophages (J774 cells) was determined, the in vitro antifungal activity of AmB-UDL was assayed. It was found that fungal strains (albicans and non-. albicans Candida ATCC strains and clinical isolates of C. albicans) were more sensitive to AmB-UDL than mammal cells. Minimum inhibitory concentration values for AmB-UDL were 5-24 and 24-50 times lower than IC50 for J774 and HaCaT cells, respectively. AmB-UDL at 1.25. μg/ml also displayed 100 and 75% anti- Leishmania braziliensis promastigote and amastigote activity, respectively. Finally, upon 1. h of non-occlusive incubation, the total accumulation of AmB in human skin was 40 times higher when applied as AmB-UDL than as AmBisome. AmB-UDL provided a profound AmB penetration toward deep epithelial layers, achieved without classical permeation enhancers. Because of that, topical treatments of cutaneous fungal infection and leishmaniasis with AmB-UDL may be regarded of potential of clinical significance.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
CANDIDA SPP.  
dc.subject
FLEXIBLE LIPOSOMES  
dc.subject
LEISHMANIA BRAZILIENSIS  
dc.subject
NANOMEDICINE  
dc.subject
SKIN DELIVERY  
dc.subject.classification
Otras Nanotecnología  
dc.subject.classification
Nanotecnología  
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS  
dc.title
Topical amphotericin B in ultradeformable liposomes: Formulation, skin penetration study, antifungal and antileishmanial activity in vitro  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-10-08T12:07:25Z  
dc.journal.volume
139  
dc.journal.pagination
190-198  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Perez, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina  
dc.description.fil
Fil: Altube, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina  
dc.description.fil
Fil: Schilrreff, Priscila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina  
dc.description.fil
Fil: Apezteguia, Gustavo Alberto. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina  
dc.description.fil
Fil: Santana Celes, Fabiana. Centro de Pesquisas Gonçalo Muniz; Brasil  
dc.description.fil
Fil: Zacchino, Susana Alicia Stella. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina  
dc.description.fil
Fil: Indiani de Oliveira, Camila. Centro de Pesquisas Gonçalo Muniz; Brasil  
dc.description.fil
Fil: Romero, Eder Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina  
dc.description.fil
Fil: Morilla, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina  
dc.journal.title
Colloids and Surfaces B: Biointerfaces  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0927776515303519  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.colsurfb.2015.12.003