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dc.contributor.author
Primo, Emiliano Nicolás
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Kogan, Marcelo Javier
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Verdejo, Hugo E.
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Bollo, Soledad
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Rubianes, María Dolores
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Rivas, Gustavo Adolfo
dc.date.available
2019-10-04T15:18:46Z
dc.date.issued
2018-07
dc.identifier.citation
Primo, Emiliano Nicolás; Kogan, Marcelo Javier; Verdejo, Hugo E.; Bollo, Soledad; Rubianes, María Dolores; et al.; Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker; American Chemical Society; ACS Applied Materials & Interfaces; 10; 28; 7-2018; 23501-23508
dc.identifier.issn
1944-8244
dc.identifier.uri
http://hdl.handle.net/11336/85208
dc.description.abstract
We report the first optical biosensor for the novel and important cardiac biomarker, galectin-3 (Gal3), using the anti-Gal3 antibody as a biorecognition element and surface plasmon resonance (SPR) for transducing the bioaffinity event. The immunosensing platform was built at a thiolated Au surface modified by self-assembling four bilayers of poly(diallyldimethylammonium chloride) and graphene oxide (GO), followed by the covalent attachment of 3-aminephenylboronic acid (3ABA). The importance of GO, both as the anchoring point of the antibody and as a field enhancer for improving the biosensor sensitivity, was critically discussed. The advantages of using 3ABA to orientate the anti-Gal3 antibody through the selective link to the Fc region were also demonstrated. The new platform represents an interesting alternative for the label-free biosensing of Gal3 in the whole range of clinically relevant concentrations (linear range between 10.0 and 50.0 ng mL-1, detection limit of 2.0 ng mL-1) with successful application for Gal3 biosensing in enriched human serum samples.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CARDIAC BIOMARKER
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GALECTIN3
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GRAPHENE OXIDE
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IMMUNOSENSOR
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LAYER-BY-LAYER SELF-ASSEMBLY
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SPR
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Química Analítica
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-09-30T18:27:26Z
dc.identifier.eissn
1944-8252
dc.journal.volume
10
dc.journal.number
28
dc.journal.pagination
23501-23508
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Primo, Emiliano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
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Fil: Kogan, Marcelo Javier. Universidad de Chile; Chile
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Fil: Verdejo, Hugo E.. Universidad de Chile; Chile. Pontificia Universidad Catolica de Chile. Facultad de Ciencias Biológicas; Chile
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Fil: Bollo, Soledad. Pontificia Universidad Católica de Chile; Chile
dc.description.fil
Fil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.description.fil
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.journal.title
ACS Applied Materials & Interfaces
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1021/acsami.8b03039
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acsami.8b03039
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