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Artículo

Isolation, taxonomic analysis, and phenotypic characterization of bacterial endophytes present in alfalfa (Medicago sativa) seeds

López, José LuisIcon ; Alvarez, FlorenciaIcon ; Principe, AnaliaIcon ; Salas, María EugeniaIcon ; Lozano, Mauricio JavierIcon ; Draghi, Walter OmarIcon ; Jofré, EdgardoIcon ; Lagares, AntonioIcon
Fecha de publicación: 02/2018
Editorial: Elsevier Science
Revista: Journal of Biotechnology
ISSN: 0168-1656
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología

Resumen

A growing body of evidence has reinforced the central role of microbiomes in the life of sound multicellular eukaryotes, thus more properly described as true holobionts. Though soil was considered a main source of plant microbiomes, seeds have been shown to be endophytically colonized by microorganisms thus representing natural carriers of a selected microbial inoculum to the young seedlings. In this work we have investigated the type of culturable endophytic bacteria that are carried within surface-sterilized alfalfa seeds. MALDI-TOF analysis revealed the presence of bacteria that belonged to 40 separate genera, distributed within four taxa (Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes). Nonsymbiotic members of the Rhizobiaceae familywere also found. The evaluation of nine different in-vitro biochemical activities demonstrated isolates with complex combinations of traits that, upon a Principal-Component-Analysis, could be classified into four phenotypicgroups. That isolates from nearly half of the genera identified had been able to colonize alfalfa plants grown under axenic conditions was remarkable. Further analyses should be addressed to investigating the colonization mechanisms of the alfalfa seeds, the evolutionary significance of the alfalfa-seed endophytes, and also how after germination the seed microbiome competes with spermospheric and rhizospheric soil bacteria to colonize newly emerging seedlings.
Palabras clave: MEDICAGO SATIVA , PLANT MICROBIOME , SEED ENDOPHYTE
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/85194
URL: http://linkinghub.elsevier.com/retrieve/pii/S0168165617317789
DOI: https://doi.org/10.1016/j.jbiotec.2017.12.020
Colecciones
Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos(IBBM)
Articulos de INST.DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Citación
López, José Luis; Alvarez, Florencia; Principe, Analia; Salas, María Eugenia; Lozano, Mauricio Javier; et al.; Isolation, taxonomic analysis, and phenotypic characterization of bacterial endophytes present in alfalfa (Medicago sativa) seeds; Elsevier Science; Journal of Biotechnology; 267; 2-2018; 55-62
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