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dc.contributor.author
Belloc, Carlos Gabriel
dc.contributor.author
Aguirre, Marisol
dc.contributor.author
Peña, Clara
dc.contributor.author
Aparicio, Jose Luis
dc.contributor.author
Duhalde Vega, Maite
dc.contributor.author
Dormois, Sarah
dc.contributor.author
Retegui, Lilia Alicia
dc.date.available
2016-11-30T18:26:08Z
dc.date.issued
2013-02
dc.identifier.citation
Belloc, Carlos Gabriel; Aguirre, Marisol; Peña, Clara; Aparicio, Jose Luis; Duhalde Vega, Maite; et al.; Properties of antibodies to a synthetic peptide representing an epitope shared by receptors of the type I cytokine family; Springer; Clinical And Experimental Medicine; 13; 1; 2-2013; 47-57
dc.identifier.issn
1591-8890
dc.identifier.uri
http://hdl.handle.net/11336/8516
dc.description.abstract
Previous works from our laboratory demonstrated that the monoclonal antibody (MAb) called R7B4 is directed to an epitope shared by various receptors corresponding to the type I cytokine receptor family, containing the common motif WSXWS or the homologous F(Y)GEFS. Later a consensus peptide significantly recognized by the MAb was identified and synthesized (sequence HGYWSEWSPE). In the present work, an homologous of the consensus sequence (HHGYWSEWSPE) was conjugated to PADRE adjuvant to produce Ab that could simulate the MAb activity, that is, acting as hormone and/or cytokine antagonists. The covalently conjugated peptide-PADRE was a better immunogen than the consensus peptide alone according to the reactivity of sera from C57BL/6 immunized mice and, besides, no Ab to PADRE were detected. Furthermore, Ab to consensus peptide elicited after peptide-PADRE inoculation into mice behaved as immunomodulatory agents, since they improved the humoral response to a foreign antigen (in this case ovalbumin). In addition, the Ab inhibited the in vitro proliferation of various cell lines, mainly cells derived from human and mouse breast cancer. Thus, immunization with the conjugate peptide-PADRE prepared under the experimental conditions described herein originated immunomodulatory Ab that, in the future, could be tested in some pathological conditions.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Receptors
dc.subject
Cytokine
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Monoclonal
dc.subject
Epitope
dc.subject.classification
Otras Ciencias de la Salud
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Ciencias de la Salud
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Properties of antibodies to a synthetic peptide representing an epitope shared by receptors of the type I cytokine family
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-11-24T17:21:36Z
dc.journal.volume
13
dc.journal.number
1
dc.journal.pagination
47-57
dc.journal.pais
Alemania
dc.journal.ciudad
Milan
dc.description.fil
Fil: Belloc, Carlos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
dc.description.fil
Fil: Aguirre, Marisol. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
dc.description.fil
Fil: Peña, Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
dc.description.fil
Fil: Aparicio, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
dc.description.fil
Fil: Duhalde Vega, Maite. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
dc.description.fil
Fil: Dormois, Sarah. Diaclone SAS; Francia
dc.description.fil
Fil: Retegui, Lilia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
dc.journal.title
Clinical And Experimental Medicine
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs10238-012-0177-6
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s10238-012-0177-6


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