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dc.contributor.author
Da Silva De Souza, Giliane
dc.contributor.author
Rodriguez, Ana Barbara F.
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Romano, Maria Isabel
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Ribeiro, Efranci Seliprandy
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Oelemann, Walter M. R.
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Da Rocha, David Gitirana
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Da Silva, Wilmar Dias
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Lasunskaia, Elena B.
dc.date.available
2019-10-02T20:26:07Z
dc.date.issued
2018-08
dc.identifier.citation
Da Silva De Souza, Giliane; Rodriguez, Ana Barbara F.; Romano, Maria Isabel; Ribeiro, Efranci Seliprandy; Oelemann, Walter M. R.; et al.; Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle; Oxford University Press; Pathogens and Disease; 76; 6; 8-2018; 1-8
dc.identifier.issn
2049-632X
dc.identifier.uri
http://hdl.handle.net/11336/85058
dc.description.abstract
Paratuberculosis (PTB) or Johne's disease is a chronic intestinal infection of ruminants, caused by Mycobacterium avium subsp. paratuberculosis. The shedding of mycobacteria in the feces starts at the initial stages and increases with disease progression, suggesting that antigens secreted by mycobacteria could be excreted in the feces. Previously, we demonstrated that the alanine and proline-rich antigen (Apa), a secretory antigen of Map, could be detected in the intestine of cows with PTB using a monoclonal antibody. In this study, we verified whether this protein can be found in consistently detectable levels in the feces of cattle with PTB. Feces were obtained from cows with Johne's disease confirmed by laboratory tests, cows with suspected PTB based on seropositivity and from PTB-free control cows. Samples were immunoprecipitated using anti-Apa monoclonal antibody and analyzed by immunoblot. The Apa was detected as a 60/70 kDa doublet band in all samples obtained from animals with laboratory-confirmed disease and in a substantial proportion of seropositive asymptomatic animals, but not in the control samples. Additionally, the antigen was detected in the feces of animals with Johne's disease by ELISA. This study strongly suggests that Apa is a potential fecal biomarker of Johne's disease that could serve for immunodiagnosis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Oxford University Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
APA PROTEIN
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FECAL BIOMARKER
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IMMUNOCHEMICAL METHODS
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PARATUBERCULOSIS
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Otras Ciencias Veterinarias
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Ciencias Veterinarias
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CIENCIAS AGRÍCOLAS
dc.title
Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-01T18:12:49Z
dc.journal.volume
76
dc.journal.number
6
dc.journal.pagination
1-8
dc.journal.pais
Reino Unido
dc.journal.ciudad
Oxford
dc.description.fil
Fil: Da Silva De Souza, Giliane. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
dc.description.fil
Fil: Rodriguez, Ana Barbara F.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
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Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Ribeiro, Efranci Seliprandy. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
dc.description.fil
Fil: Oelemann, Walter M. R.. Universidade Federal do Rio de Janeiro; Brasil
dc.description.fil
Fil: Da Rocha, David Gitirana. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
dc.description.fil
Fil: Da Silva, Wilmar Dias. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
dc.description.fil
Fil: Lasunskaia, Elena B.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
dc.journal.title
Pathogens and Disease
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femspd/article/76/6/fty063/5057472
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1093/femspd/fty063
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