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dc.contributor.author
Jalalvand, Ali R.  
dc.contributor.author
Ghobadi, Sirous  
dc.contributor.author
Goicoechea, Hector Casimiro  
dc.contributor.author
Gu, Hui Wen  
dc.contributor.author
Sanchooli, Esmael  
dc.date.available
2019-10-01T13:50:40Z  
dc.date.issued
2018-10  
dc.identifier.citation
Jalalvand, Ali R.; Ghobadi, Sirous; Goicoechea, Hector Casimiro; Gu, Hui Wen; Sanchooli, Esmael; Investigation of interactions of Comtan with human serum albumin by mathematically modeled voltammetric data: A study from bio-interaction to biosensing; Elsevier Science Sa; Bioelectrochemistry; 123; 10-2018; 162-172  
dc.identifier.issn
1567-5394  
dc.identifier.uri
http://hdl.handle.net/11336/84886  
dc.description.abstract
In this work, voltammetric data recorded at a glassy carbon electrode (GCE) were separately used to investigate the interactions of entacapone (Comtan, CAT) with human serum albumin (HSA). Then, an augmented data matrix was constructed by the combination of voltammetric and spectroscopic data and simultaneously analysed by multivariate curve resolution-alternating least squares (MCR-ALS) to obtain more information about CAT-HSA interactions. The absence of rotational ambiguities in results obtained by MCR-ALS was verified with the help of MCR-BANDS and we confirmed that the results were unambiguous and reliable. Binding of CAT to HSA was also modeled by molecular docking and the results were compatible with those of obtained by recording experimental data. Hard-modeling of combined voltammetric and spectroscopic data by EQUISPEC as an efficient chemometric algorithm helped us to compute binding constant of CAT-HSA complex specie which was in a good agreement with the binding constant value obtained by direct analysis of experimental data. For electrochemical sensing of serum albumin two amperometric measurements were performed to determine HSA in 2–27 nM and 27–70 nM with a limit of detection of 0.51 nM and a sensitivity of 1.84 μA nM−1.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science Sa  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
COMTAN  
dc.subject
HUMAN SERUM ALBUMIN  
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INTERACTION  
dc.subject.classification
Química Analítica  
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Ciencias Químicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Investigation of interactions of Comtan with human serum albumin by mathematically modeled voltammetric data: A study from bio-interaction to biosensing  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-09-27T15:01:04Z  
dc.journal.volume
123  
dc.journal.pagination
162-172  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Jalalvand, Ali R.. Kermanshah University of Medical Sciences; Irán  
dc.description.fil
Fil: Ghobadi, Sirous. Razi University; Irán  
dc.description.fil
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina  
dc.description.fil
Fil: Gu, Hui Wen. Yangtze University; China  
dc.description.fil
Fil: Sanchooli, Esmael. University of Zabol; Irán  
dc.journal.title
Bioelectrochemistry  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bioelechem.2018.05.008  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1567539418301622