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dc.contributor.author
Micheloud, Gabriela Analia
dc.contributor.author
Gioria, Verónica Viviana
dc.contributor.author
Pérez, Gustavo
dc.contributor.author
Claus, Juan Daniel
dc.date.available
2019-09-29T20:39:24Z
dc.date.issued
2009-01
dc.identifier.citation
Micheloud, Gabriela Analia; Gioria, Verónica Viviana; Pérez, Gustavo; Claus, Juan Daniel; Production of occlusion bodies of Anticarsia gemmatalis multiple nucleopolyhedrovirus in serum-free suspension cultures of the saUFL-AG-286 cell line: Influence of infection conditions and statistical optimization; Elsevier Science; Journal of Virological Methods; 162; 1-2; 1-2009; 258-266
dc.identifier.issn
0166-0934
dc.identifier.uri
http://hdl.handle.net/11336/84790
dc.description.abstract
The influence of the conditions of infection on the yield of occlusion bodies (OBs) of the Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV), produced in serum-free suspension cultures of saUFL-AG-286 cells, was investigated by two 22 full factorial experiments with centre point. Each experiment tested the effects of the initial cell density and the multiplicity of infection at two levels, in the four possible combinations of levels and conditions, plus a further combination with each condition set at the middle of its extreme levels. The yield of occlusion bodies proved to be sensitive to the modification of infection conditions. Maximum yield as high as 3 × 108 OBs mL-1 was attained provided that the maximum density of viable cells was in the range between 4 and 8 × 105 cells mL-1. The optimum value of the maximum density of viable cells could be reached by the combination of several values of initial cell density and multiplicity of infection. A regression model was established and validated in order to optimize the infection conditions. These results demonstrate the importance of an adequate selection of infection conditions, and they could be useful in the development of a feasible in vitro process to produce the AgMNPV insecticide in a new serum-free medium.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ANTICARSIA GEMMATALIS MULTIPLE NUCLEOPOLYHEDROVIRUS
dc.subject
SAUFLA-AG-286 CELL LINE
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SERUM-FREE MEDIUM
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INFECTION CONDITIONS
dc.subject.classification
Virología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Production of occlusion bodies of Anticarsia gemmatalis multiple nucleopolyhedrovirus in serum-free suspension cultures of the saUFL-AG-286 cell line: Influence of infection conditions and statistical optimization
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-09-27T14:16:40Z
dc.journal.volume
162
dc.journal.number
1-2
dc.journal.pagination
258-266
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Micheloud, Gabriela Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil
Fil: Gioria, Verónica Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil
Fil: Pérez, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo y Diseño. Universidad Tecnológica Nacional. Facultad Regional Santa Fe. Instituto de Desarrollo y Diseño; Argentina
dc.description.fil
Fil: Claus, Juan Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.journal.title
Journal of Virological Methods
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jviromet.2009.08.021
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