Artículo
Cloning, Expression, Purification And Physical And Kinetic Characterization Of The Phosphoenolpyruvate Carboxylase From Orange (Citrus sinensis Osbeck var. Valencia) Fruit Juice Sacs
Perotti, Valeria Elisa
; Figueroa, Carlos Maria
; Andreo, Carlos Santiago
; Iglesias, Alberto Alvaro
; Podesta, Florencio Esteban
Fecha de publicación:
11/2010
Editorial:
Elsevier
Revista:
Plant Science
ISSN:
0168-9452
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Phosphoenolpyruvate (PEP) carboxylase (PEPCase) from orange fruit juice sacs has been cloned and heterogously expressed in high yield. The purified recombinant enzyme displays properties typical of plant PEPCase, including activation by sugar phosphates and inhibition by malate and citrate. Malate inhibition is weak in the physiological pH range, and the enzyme is also poorly affected by Glu and Asp, known inhibitors of C3 plants PEPCases. However, it is strongly inhibited by citrate. Orange fruit PEPCase phosphorylation by mammalian protein kinase A decreased inhibition by malate. The enzyme presents an unusual high molecular mass in the absence of PEP, while in its presence it displays a more common tetrameric arrangement. The overall properties of the enzyme suggest that it is suited for organic acid synthesis and NADH reoxidation in the mature fruit. The present study provides the first analysis of a recombinant fruit PEPCase.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - ROSARIO)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Articulos(CEFOBI)
Articulos de CENTRO DE EST.FOTOSINTETICOS Y BIOQUIMICOS (I)
Articulos de CENTRO DE EST.FOTOSINTETICOS Y BIOQUIMICOS (I)
Articulos(IAL)
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Citación
Perotti, Valeria Elisa; Figueroa, Carlos Maria; Andreo, Carlos Santiago; Iglesias, Alberto Alvaro; Podesta, Florencio Esteban; Cloning, Expression, Purification And Physical And Kinetic Characterization Of The Phosphoenolpyruvate Carboxylase From Orange (Citrus sinensis Osbeck var. Valencia) Fruit Juice Sacs; Elsevier; Plant Science; 179; 5; 11-2010; 527-535
Compartir
Altmétricas