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dc.contributor.author
Rashidi, Khodabakhsh
dc.contributor.author
Mahmoudi, Majid
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Mohammadi, Ghobad
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Zangeneh, Mohammad Mahdi
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Korani, Shahla
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Goicoechea, Hector Casimiro
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dc.contributor.author
Gu, Hui-Wen
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Jalalvand, Ali R.
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dc.date.available
2019-09-28T00:26:53Z
dc.date.issued
2018-12
dc.identifier.citation
Rashidi, Khodabakhsh; Mahmoudi, Majid; Mohammadi, Ghobad; Zangeneh, Mohammad Mahdi; Korani, Shahla; et al.; Simultaneous co-immobilization of three enzymes onto a modified glassy carbon electrode to fabricate a high-performance amperometric biosensor for determination of total cholesterol; Elsevier Science; International Journal of Biological Macromolecules; 120; Part A; 12-2018; 587-595
dc.identifier.issn
0141-8130
dc.identifier.uri
http://hdl.handle.net/11336/84761
dc.description.abstract
In this work, we have fabricated a novel amperometric cholesterol (CHO) biosensor because of the importance of determination of CHO levels in blood which is an important parameter for diagnosis and prevention of disease. To achieve this goal, cholesterol oxidase, cholesterol esterase and horseradish peroxidase were simultaneously co-immobilized onto a glassy carbon electrode (GCE) modified with gold nanoparticles/chitin-ionic liquid/poly(3,4-ethylenedioxypyrrole)/graphene-multiwalled carbon nanotubes-1,1′-ferrocenedicarboxylic acid-ionic liquid. Modifications applied to the bare GCE were characterized by cyclic voltammetry, electrochemical impedance spectroscopy and scanning electron microscopy. The biosensor detected CHO in linear ranges of 0.1–25 μM and 25–950 μM with a detection limit of 0.07 μM. The sensitivity of the biosensor was estimated to be 6.6 μA μM−1 cm−2, its response time was <5 s and Michaelis-Menten constant was calculated to be 0.12 μM. Results obtained in this study revealed that the biosensor was selective, sensitive, stable, repeatable and reproducible. Finally, the biosensor was successfully applied to the determination of CHO levels in rats plasma.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BIOSENSOR
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CHOLESTEROL
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RATS PLASMA
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Química Analítica
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dc.subject.classification
Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
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dc.title
Simultaneous co-immobilization of three enzymes onto a modified glassy carbon electrode to fabricate a high-performance amperometric biosensor for determination of total cholesterol
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-09-27T15:01:00Z
dc.journal.volume
120
dc.journal.number
Part A
dc.journal.pagination
587-595
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Rashidi, Khodabakhsh. Kermanshah University of Medical Sciences; Irán
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Fil: Mahmoudi, Majid. Kermanshah University of Medical Sciences; Irán
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Fil: Mohammadi, Ghobad. Kermanshah University of Medical Sciences; Irán
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Fil: Zangeneh, Mohammad Mahdi. Kermanshah University of Medical Sciences; Irán. Razi University,; Irán
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Fil: Korani, Shahla. Kermanshah University of Medical Sciences; Irán
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Fil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
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Fil: Gu, Hui-Wen. Yangtze University; China
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Fil: Jalalvand, Ali R.. Kermanshah University of Medical Sciences; Irán
dc.journal.title
International Journal of Biological Macromolecules
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0141813018330186
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ijbiomac.2018.08.163
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