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dc.contributor.author
Cirigliano, Adriana Monica
dc.contributor.author
Cabrera, Gabriela Myriam
dc.date.available
2019-09-27T20:54:16Z
dc.date.issued
2014-03
dc.identifier.citation
Cirigliano, Adriana Monica; Cabrera, Gabriela Myriam; Differentiation of cyclosporin A from isocyclosporin A by liquid chromatography/ electrospray ionization mass spectrometry with post-column addition of divalent metal salt; John Wiley & Sons Ltd; Rapid Communications in Mass Spectrometry ; 28; 5; 3-2014; 465-470
dc.identifier.issn
0951-4198
dc.identifier.uri
http://hdl.handle.net/11336/84728
dc.description.abstract
RATIONALE Cyclosporin A (CsA) rearranges to its isomer isocyclosporin A (isoCsA) upon acid hydrolysis and also during ionization in the ion source of the mass spectrometer. It has been reported that both compounds could not be differentiated by tandem mass spectrometry (MS/MS) using atmospheric pressure ionization (API) sources and ambiguously differentiated by using other sources. In order to analyze these compounds which are common fungal metabolites, it is relevant to develop a simple method for their differentiation. METHODS CsA and isoCsA were analyzed by liquid chromatography/mass spectrometry (LC/MS) with post-column addition of metal ion solutions in a quadrupole time-of-flight instrument equipped with an electrospray ionization (ESI) source. RESULTS Mass spectra of CsA obtained upon post-column addition of solutions of Ca(II), Cu(II) and Zn(II) showed complexes between cyclosporin and the metal, including [2CsA + Me]2+ and [CsA-H + Me]+. These complexes were not observed in the spectra of isoCsA. The same results were observed at different metal concentrations. CONCLUSIONS Differentiation via metal complexation in positive ion mode LC/ESI-MS was performed to simultaneously distinguish CsA and its isomer isoCsA.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
John Wiley & Sons Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CYCLOSPORINE
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MASS SPECTROMETRY
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ELECTROSPRAY IONIZATION
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DIVALENTS CATIONS
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Química Orgánica
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Differentiation of cyclosporin A from isocyclosporin A by liquid chromatography/ electrospray ionization mass spectrometry with post-column addition of divalent metal salt
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-09-27T17:09:22Z
dc.journal.volume
28
dc.journal.number
5
dc.journal.pagination
465-470
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Cirigliano, Adriana Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina
dc.description.fil
Fil: Cabrera, Gabriela Myriam. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina
dc.journal.title
Rapid Communications in Mass Spectrometry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/rcm.6805
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/rcm.6805
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