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dc.contributor.author
Matzkin, Maria Eugenia
dc.contributor.author
Pellizari, Eliana Herminia
dc.contributor.author
Rossi, Soledad Paola
dc.contributor.author
Calandra, Ricardo Saul
dc.contributor.author
Cigorraga, Selva Beatriz
dc.contributor.author
Frungieri, Monica Beatriz
dc.date.available
2016-11-29T20:21:37Z
dc.date.issued
2012-11
dc.identifier.citation
Matzkin, Maria Eugenia; Pellizari, Eliana Herminia; Rossi, Soledad Paola; Calandra, Ricardo Saul; Cigorraga, Selva Beatriz; et al.; Exploring the cyclooxygenase 2 (COX2)/15d delta12,14PGJ2 system in hamster Sertoli cells: regulation by FSH/testosterone and relevance to glucose uptake; Elsevier; General And Comparative Endocrinology; 179; 2; 11-2012; 254-264
dc.identifier.issn
0016-6480
dc.identifier.uri
http://hdl.handle.net/11336/8470
dc.description.abstract
We have previously described a stimulatory effect of testosterone on cyclooxygenase 2 (COX2) expression and prostaglandin (PG) synthesis, and the involvement of PGs in the modulation of testosterone production in Leydig cells of the seasonal breeder Syrian hamster. In this study, we investigated the existence of a COX2/PGs system in hamster Sertoli cells, its regulation by testosterone and FSH, and its effect on glucose uptake. COX2 expression was observed in Sertoli cells of both reproductively active and inactive adult hamsters. Testosterone and the plasma membrane-impermeable testosterone-BSA significantly induced COX2 expression, mitogen activated protein kinases 1/2 (MAPK1/2) phosphorylation and 15d-delta12,14PGJ2 production in Sertoli cells purified from photoperiodically regressed hamsters. These actions were abolished by the antiandrogen bicalutamide and by the inhibitor of MAPK kinase (MEK1/2) U0126, suggesting that testosterone exerts its stimulatory effect on COX2/PGs through a non-classical mechanism that involves the presence of androgen receptors and MAPK1/2 activation. FSH also stimulated COX2/PGs via MAPK1/2 phosphorylation. FSH and testosterone stimulate, whereas 15d-delta12,14PGJ2 via PPAR?× inhibits, [2,6-3H]-2-deoxy-D-glucose ([3H]-2-DOG) uptake. Meloxicam, a selective COX2 inhibitor, further increases [3H]-2-DOG uptake in the presence of FSH or testosterone. Thus, in addition to their positive effect, FSH and testosterone may also exert an indirect negative regulation on glucose uptake which involves the COX2/15d-delta12,14PGJ2/PPAR?× system. Overall, these results demonstrate the presence of a COX2/PG system in hamster Sertoli cells which might act as a local modulator of FSH and testosterone actions.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cyclooxygenase 2
dc.subject
Testosterone
dc.subject
Fsh
dc.subject
Sertoli Cells
dc.subject
Hamsters
dc.subject.classification
Otras Medicina Básica
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Exploring the cyclooxygenase 2 (COX2)/15d delta12,14PGJ2 system in hamster Sertoli cells: regulation by FSH/testosterone and relevance to glucose uptake
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-11-22T21:22:01Z
dc.identifier.eissn
1095-6840
dc.journal.volume
179
dc.journal.number
2
dc.journal.pagination
254-264
dc.journal.pais
Estados Unidos
dc.journal.ciudad
New York
dc.description.fil
Fil: Matzkin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
dc.description.fil
Fil: Pellizari, Eliana Herminia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.description.fil
Fil: Rossi, Soledad Paola. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Calandra, Ricardo Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Cigorraga, Selva Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.description.fil
Fil: Frungieri, Monica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
dc.journal.title
General And Comparative Endocrinology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ygcen.2012.08.020
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0016648012003486


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  • Articulos(IBYME) [1159]
    Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
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    Articulos de CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"

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