Temperature benefits the photosynthetic performance of the diatoms Chaetoceros gracilis and Thalassiosira weissflogii when exposed to UVR

Abstract

The aim of this study was to assess the combined effects of temperature and UVR on the photosynthesis performance of two diatoms - Chaetoceros gracilis and Thalassiosira weissflogii. In particular, we evaluated the role of UVR in inducing photoinhibition and the potential mitigation of this negative effect by an increase in temperature. Cultures were pre-acclimated at two temperatures - 18 °C and 23 °C - and exposed to different radiation treatments - UVR. +. PAR (280-700 nm); UV-A. +. PAR (315-700 nm) and PAR only (400-700 nm) under two temperatures: 18 °C (local surface summer water temperature) and 23 °C (simulating a potential increase estimated by the year 2100). Exposure to natural solar radiation resulted in UVR-induced photoinhibition that was significantly higher in T. weissflogii than in C. gracilis. Both species benefited from the higher temperature (23 °C) resulting in a lower photoinhibition as compared to samples exposed at 18 °C. Inter-specific differences were determined in regard to the heat dissipation processes (NPQ) which were higher at high temperatures, and much more evident in C. gracilis than in T. weissflogii. The analyses of inhibition and recovery rates under different irradiances indicate that the balance between negative (inhibition) and positive (repair-dissipation) effects shifted towards a more positive balance with increasing temperature. Our results highlight for a beneficial effect of temperature on photosynthesis performance during exposure to UVR, although important inter-specific differences are found, probably due to differences in cell size as well as in their distribution within the oceanic realm (i.e., coastal versus oceanic species).