Mostrar el registro sencillo del ítem
dc.contributor.author
Stanganelli, Carmen Graciela
dc.contributor.author
Travella, Ana Carolina
dc.contributor.author
Bezares, Raimundo F.
dc.contributor.author
Slavutsky, Irma Rosa
dc.date.available
2019-09-26T22:14:18Z
dc.date.issued
2013-08
dc.identifier.citation
Stanganelli, Carmen Graciela; Travella, Ana Carolina; Bezares, Raimundo F.; Slavutsky, Irma Rosa; Immunoglobulin gene rearrangements and mutational status in argentinian patients with chronic lymphocytic leukemia; Elsevier Inc.; Clinical Lymphoma, Myeloma and Leukemia; 13; 4; 8-2013; 447-457
dc.identifier.issn
2152-2650
dc.identifier.uri
http://hdl.handle.net/11336/84634
dc.description.abstract
Background: Chronic lymphocytic leukemia (CLL) is a clinically heterogeneous disease. The mutational status of the immunoglobulin heavy chain variable (IGHV) region represents one of the best prognostic markers and defines 2 disease subgroups: mutated (M-CLL) and unmutated (UM-CLL), with different clinical course. Materials and Methods: IGHV-D-J gene rearrangements and mutational status were analyzed in 73 Argentinian patients with CLL, 22 previously treated, by reverse transcriptase-polymerase chain reaction and bidirectional sequencing. The results were compared with those reported in other geographic regions. Fluorescence in situ hybridization analysis was also performed. Results: A total of 43 (58.9%) cases were of patients with M-CLL, and 30 (41.1%) were patients with UM-CLL. Deletion of chromosome 13q14 as a single alteration was more frequently observed in the M-CLL group (48%) than in the UM-CLL group (24%). In the M-CLL group, the proportion of cases with deletion of chromosome 13q14 was significantly higher than those with +12 and those with deletions of chromosomes 17p and 11q (P =.003). The most frequently used IGHV families were IGHV3 > IGHV1 > IGHV4, which are different from those observed in Asian, Brazilian, and Uruguayan series. The IGHV3-23 gene (10.8%) was the most commonly used, followed by IGHV1-69 (9.5%), IGHV4-59 and IGHV2-5 (6.8% each), and IGHV3-21 and IGHV3-30 (5.4% each). IGHV4-34 showed the lowest frequency (2.7%) in our cohort compared with published data, whereas IGHV4-59, IGHV3-72, and IGHV2-5 were overexpressed in our series. Stereotyped HCDR3 (heavy chain complementary determining region 3) was found in 9.5% of patients. Conclusions: Our results showed that Argentinian patients with CLL display an IGHV gene usage that resembles that observed in Western countries and exhibited interesting similarities and differences with respect to published series from other Latin American populations, which reflect variations in the genetic background.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Inc.
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CHRONIC LYMPHOCYTIC LEUKEMIA
dc.subject
FLUORESCENCE IN SITU HYBRIDIZATION
dc.subject
IMMUNOGLOBULIN HEAVY CHAIN VARIABLE GENE
dc.subject
SOMATIC HYPERMUTATION
dc.subject
STEREOTYPED B-CELL RECEPTOR
dc.subject.classification
Hematología
dc.subject.classification
Medicina Clínica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Immunoglobulin gene rearrangements and mutational status in argentinian patients with chronic lymphocytic leukemia
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-06-26T13:27:51Z
dc.identifier.eissn
2152-2669
dc.journal.volume
13
dc.journal.number
4
dc.journal.pagination
447-457
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Stanganelli, Carmen Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
dc.description.fil
Fil: Travella, Ana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
dc.description.fil
Fil: Bezares, Raimundo F.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Dr. Teodoro Álvarez"; Argentina
dc.description.fil
Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
dc.journal.title
Clinical Lymphoma, Myeloma and Leukemia
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2152265013000487
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.clml.2013.02.019
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.clinical-lymphoma-myeloma-leukemia.com/article/S2152-2650(13)00048-7/abstract
Archivos asociados