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dc.contributor.author
Ambroggio, Maria Belen
dc.contributor.author
Perrig, Melina Soledad
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Camussone, Cecilia María
dc.contributor.author
Pujato, Nazarena
dc.contributor.author
Bertón, Alicia
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Gianneechini, Edgardo
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Alvarez, Silvia
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Marcipar, Iván Sergio
dc.contributor.author
Calvinho, Luis Fernando
dc.contributor.author
Barbagelata, María Sol
dc.date.available
2019-09-26T19:16:10Z
dc.date.issued
2018-08
dc.identifier.citation
Ambroggio, Maria Belen; Perrig, Melina Soledad; Camussone, Cecilia María; Pujato, Nazarena; Bertón, Alicia; et al.; Survey of potential factors involved in the low frequency of CP5 and CP8 expression in Staphylococcus aureus isolates from mastitis of dairy cattle from Argentina, Chile, and Uruguay; Polish Academy of Sciences; Journal Of Applied Genetics; 59; 3; 8-2018; 357-363
dc.identifier.issn
1234-1983
dc.identifier.uri
http://hdl.handle.net/11336/84570
dc.description.abstract
Staphylococcus aureus produces capsular polysaccharides (CPs) both in vivo and under defined culture conditions being serotypes 5 and 8 the most prevalent. S. aureus isolates that fail to produce CP5 or CP8 are defined as non-typeable (NT). Loss of capsule expression, however, may lead to S. aureus persistence in a chronically infected host. The prevalence of NT strains of S. aureus isolated from bovine mastitis varies according to the geographic origin of the strain. The aims of this work were to detect phenotypically and genotypically the capsular profile of 144 S. aureus isolated from bovine mastitis in Argentina, Chile, and Uruguay and explore the factors that are considered to be associated with capsule expression as presence of IS257, IScap, and agr typing of non-related collection. The detection of the IS257, IScap, cap genes, and agr typing was performed using PCR. The detection and quantification of capsular polysaccharide production were performed by ELISA assays. We found that 96% of the S. aureus isolates investigated carried cap5(8) genes but over 75% of strains do not express capsule in the three countries studied. However, only 6 isolates from Argentina carried the IScap element that totally suppressed the expression of the capsule, suggesting that other factors could influence on CP expression. Moreover, the agrI/NT association was statistically significant suggesting that this profile is a phenomenon observed not only in other parts of the world but also in our region.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Polish Academy of Sciences
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
AGR TYPING
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BOVINE MASTITIS
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CAPSULE EXPRESSION
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ISCAP
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STAPHYLOCOCCUS AUREUS
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Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Survey of potential factors involved in the low frequency of CP5 and CP8 expression in Staphylococcus aureus isolates from mastitis of dairy cattle from Argentina, Chile, and Uruguay
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-08-08T18:09:39Z
dc.journal.volume
59
dc.journal.number
3
dc.journal.pagination
357-363
dc.journal.pais
Polonia
dc.journal.ciudad
Bialowieza
dc.description.fil
Fil: Ambroggio, Maria Belen. Universidad Nacional del Litoral; Argentina
dc.description.fil
Fil: Perrig, Melina Soledad. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
dc.description.fil
Fil: Camussone, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
dc.description.fil
Fil: Pujato, Nazarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral; Argentina
dc.description.fil
Fil: Bertón, Alicia. Lactodiagnóstico Sur Uruguay; Uruguay
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Fil: Gianneechini, Edgardo. Laboratory Veterinary Direction “Miguel C. Rubino”; Uruguay
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Fil: Alvarez, Silvia. Udder Health; Chile
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Fil: Marcipar, Iván Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral; Argentina
dc.description.fil
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil
Fil: Barbagelata, María Sol. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
dc.journal.title
Journal Of Applied Genetics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1007/s13353-018-0443-8
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs13353-018-0443-8
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