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dc.contributor.author
Zuo, P.
dc.contributor.author
Albrecht, T.
dc.contributor.author
Barker, P.D.
dc.contributor.author
Murgida, Daniel Horacio
dc.contributor.author
Hildebrandt, P.
dc.date.available
2019-09-26T19:08:33Z
dc.date.issued
2009-12
dc.identifier.citation
Zuo, P.; Albrecht, T.; Barker, P.D.; Murgida, Daniel Horacio; Hildebrandt, P.; Interfacial redox processes of cytochrome b562; Royal Society of Chemistry; Physical Chemistry Chemical Physics; 11; 34; 12-2009; 7430-7436
dc.identifier.issn
1463-9076
dc.identifier.uri
http://hdl.handle.net/11336/84561
dc.description.abstract
The anionic soluble heme protein cytochrome b562 was electrostatically immobilised on Ag electrodes coated with positively charged self-assembled monolayers of amino-terminated alkanethiols. The structure of the heme pocket, the redox equilibria, and the electron transfer dynamics were studied by stationary and time-resolved surface enhanced resonance Raman spectroscopy, complemented by cyclic voltammetry measurements of the interfacial redox process. The conformational and redox equilibria of the immobilised protein are compared to those of the cationic heme protein cytochrome c immobilised on negatively charged electrode coatings. Similarities and differences can be rationalised in terms of the respective electric fields at the interfaces of amino- and carboxyl-terminated electrode coatings. The heterogeneous electron transfer rate of cytochrome b562 only slightly increases with decreasing thickness from ca. 20 to 11 Å, implying that the electron tunneling is not the rate-limiting step. In contrast to cytochrome c on carboxyl-terminated monolayers, this behaviour cannot be attributed to protein re-orientation gating the heterogeneous electron transfer. Instead, it may reflect the interplay between interprotein electron transfer and heterogeneous electron transfer via protein orientations exhibiting particularly high tunneling probabilities for the electron exchange with the electrode.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Royal Society of Chemistry
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Citocromos
dc.subject
Transferencia Electrónica
dc.subject
Raman
dc.subject.classification
Físico-Química, Ciencia de los Polímeros, Electroquímica
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Interfacial redox processes of cytochrome b562
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-03-27T17:53:58Z
dc.journal.volume
11
dc.journal.number
34
dc.journal.pagination
7430-7436
dc.journal.pais
Reino Unido
dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Zuo, P.. Technishe Universitat Berlin; Alemania
dc.description.fil
Fil: Albrecht, T.. Imperial College London; Reino Unido
dc.description.fil
Fil: Barker, P.D.. University of Cambridge; Reino Unido
dc.description.fil
Fil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina
dc.description.fil
Fil: Hildebrandt, P.. Technishe Universitat Berlin; Alemania
dc.journal.title
Physical Chemistry Chemical Physics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2009/cp/b904926f
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1039/B904926F
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