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dc.contributor.author
Trasorras, Virginia Luz
dc.contributor.author
Baca Castex, Clara
dc.contributor.author
Alonso, Ana Elisa
dc.contributor.author
Giuliano, Susana María
dc.contributor.author
Santa Cruz, Romina
dc.contributor.author
Arraztoa, Claudia Cecilia
dc.contributor.author
Chaves, María Graciela
dc.contributor.author
Rodriguez, Diana Isabel
dc.contributor.author
Neild, Debora Margarita
dc.contributor.author
Miragaya, Marcelo
dc.date.available
2019-09-26T15:39:22Z
dc.date.issued
2014-07
dc.identifier.citation
Trasorras, Virginia Luz; Baca Castex, Clara; Alonso, Ana Elisa; Giuliano, Susana María; Santa Cruz, Romina; et al.; First llama (Lama glama) pregnancy obtained after in vitro fertilization and in vitro culture of gametes from live animals; Elsevier Science; Animal Reproduction Science; 148; 1-2; 7-2014; 83-89
dc.identifier.issn
0378-4320
dc.identifier.uri
http://hdl.handle.net/11336/84527
dc.description.abstract
The aim of this study was to evaluate the developmental competence and pregnancy rate of llama hatched blastocysts produced in vitro using gametes from live animals and two different culture conditions. Fifteen adult females were superstimulated with 1500IU of eCG, eleven (73%) responded to the treatment and were used as oocyte donors. Follicular aspiration was conducted by flank laparotomy. Semen collections were performed under general anesthesia by electroejaculation of the male. Sixty-six COCs were recovered from 77 aspirated follicles (86% recovery) and were randomly placed in Fertil-TALP microdroplets with the sperm suspension (20×106live spermatozoa/ml). After 24h, they were placed in SOFaa medium supplemented with FCS and randomly assigned to one of two culture conditions. Culture condition 1 (CC1) consisted of 6 days of culture (n=28) and culture condition 2 (CC2) consisted of renewing the culture medium every 48h (n=35). In CC1, the blastocyst rate was 36% (10/28) and the hatched blastocyst rate was 28% (8/28) whereas in CC2, the blastocyst rate was 34% (12/35) and the hatched blastocyst rate was 20% (7/35) (p>0.05). No pregnancies were obtained after embryo transfer (ET) of CC1 blastocysts (0/8) while one pregnancy was obtained (1/7) after transferring a hatched blastocyst from CC2. Forty-two days after the ET, the pregnancy was lost.This study represents the first report of a pregnancy in the llama after intrauterine transfer of embryos produced by in vitro fertilization using gametes from live animals.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
EMBRYO TRANSFER
dc.subject
IVC
dc.subject
IVF
dc.subject
LLAMA
dc.subject
PREGNANCY
dc.subject
SOF
dc.subject.classification
Ciencias Veterinarias
dc.subject.classification
Ciencias Veterinarias
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
First llama (Lama glama) pregnancy obtained after in vitro fertilization and in vitro culture of gametes from live animals
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-09-24T12:51:31Z
dc.journal.volume
148
dc.journal.number
1-2
dc.journal.pagination
83-89
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Trasorras, Virginia Luz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Baca Castex, Clara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Alonso, Ana Elisa. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Giuliano, Susana María. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Santa Cruz, Romina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
dc.description.fil
Fil: Arraztoa, Claudia Cecilia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Chaves, María Graciela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
dc.description.fil
Fil: Rodriguez, Diana Isabel. Universidad de Buenos Aires. Facultad de Cs.veterinarias. Departamento de Medicina; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
dc.description.fil
Fil: Neild, Debora Margarita. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
dc.description.fil
Fil: Miragaya, Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
dc.journal.title
Animal Reproduction Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.anireprosci.2014.04.006
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378432014001158


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