Artículo
Set-up of an infrared fast behavioral assay using zebrafish (Danio rerio) larvae, and its application in compound biotoxicity screening
Bichara, Darío Román
; Calcaterra, Nora Beatriz
; Arranz, Silvia Eda; Armas, Pablo
; Simonetta, Sergio Hernan
Fecha de publicación:
02/2014
Editorial:
John Wiley & Sons Ltd
Revista:
Journal of Applied Toxicology
ISSN:
0260-437X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Zebrafish (Danio rerio) is increasingly employed for evaluating toxicity and drug discovery assays. Commonly experimental approaches for biotoxicity assessment are based on visual inspection or video recording. However, these techniques are limited for large-scale assays, as they demand either a time-consuming detailed inspection of the animals or intensive computing resources in order to analyze a considerable amount of screenshots. Recently, we have developed a simple methodology for tracking the locomotor activity of small animals cultured in microtiter plates. In this work, we implemented this automatic methodology, based on infrared (IR) microbeam scattering, for measuring behavioral activity in zebrafish larvae. We determined the appropriate culture conditions, number of animals and stage of development to get robust results. Furthermore, we validated this methodology as a rapid test for evaluating toxicity. By measuring the effects of reference compounds on larvae activity, we were able to estimate the concentration that could cause a 50% decrease in activity events values (AEC 50 ), showing a strong linear correlation (R 2 =0.91) with the LC 50 values obtained with the standard DarT test. The toxicity order of the measured compounds was CuSO 4 >2,4-dinitrophenol>3,4-dichloroaniline>SDS>sodium benzoate>EDTA>K 2 CrO 4 ; regarding solvents, EtOH≈DMSO. In this study, we demonstrate that global swimming behavior could be a simple readout for toxicity, easy to scale-up in automated experiments. This approach is potentially applicable for fast ecotoxicity assays and whole-organism high-throughput compound screening, reducing the time and money required to evaluate unknown samples and to identify leading pharmaceutical compounds.
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Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos(IIBBA)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Citación
Bichara, Darío Román; Calcaterra, Nora Beatriz; Arranz, Silvia Eda; Armas, Pablo; Simonetta, Sergio Hernan; Set-up of an infrared fast behavioral assay using zebrafish (Danio rerio) larvae, and its application in compound biotoxicity screening; John Wiley & Sons Ltd; Journal of Applied Toxicology; 34; 2; 2-2014; 214-219
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