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dc.contributor.author
Costa, Juan Gabriel
dc.contributor.author
Duré, Andrea Belén
dc.date.available
2019-09-25T18:49:58Z
dc.date.issued
2016-11
dc.identifier.citation
Costa, Juan Gabriel; Duré, Andrea Belén; Immunochemical evaluation of two Toxoplasma gondii GRA8 sequences to detect acute toxoplasmosis infection; Academic Press Ltd - Elsevier Science Ltd; Microbial Pathogenesis; 100; 11-2016; 229-236
dc.identifier.issn
0882-4010
dc.identifier.uri
http://hdl.handle.net/11336/84430
dc.description.abstract
In this work, two Toxoplasma gondii GRA8 protein sequences were tested by indirect ELISA and measurement of avidity to differentiate between acute and chronic toxoplasmosis infection. Using the antigen called GRA8B, 79.7% sensitivity and 84.1% specificity was achieved detecting IgG concentrations and a 71.2% sensitivity and a 68.3% specificity detecting IgA concentrations. This study is the first to report IgA detection with GRA8 by ELISA to differentiate stages of infection. Unfortunately the indirect ELISA to detect IgM was not effective in distinguishing stages. Also, this work is the first to report that the GRA8 protein can aid the differentiation between acute and chronic phase infection by measuring IgG antibody avidity, a technique in which we obtained 85.71% and 100% of sensitivity and specificity, respectively. Finally, in silico tools were used to explain the differences in our immunochemistry results.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Ltd - Elsevier Science Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ACUTE INFECTION
dc.subject
ANTIGEN
dc.subject
BIOINFORMATICS
dc.subject
GRA8
dc.subject
SERODIAGNOSIS
dc.subject
TOXOPLASMOSIS
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
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Inmunología
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Immunochemical evaluation of two Toxoplasma gondii GRA8 sequences to detect acute toxoplasmosis infection
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-09-23T17:10:37Z
dc.journal.volume
100
dc.journal.pagination
229-236
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Costa, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Santa Fe. Ministerio de Salud y Medio Ambiente - Rosario. Hospital Provincial del Centenario; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
dc.description.fil
Fil: Duré, Andrea Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; Argentina
dc.journal.title
Microbial Pathogenesis
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0882401016302042
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.micpath.2016.09.021
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