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dc.contributor.author
Worley, Jay N.
dc.contributor.author
Pombo, Marina Alejandra
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dc.contributor.author
Zheng, Yi
dc.contributor.author
Dunham, Diane M.
dc.contributor.author
Myers, Christopher R.
dc.contributor.author
Fei, Zhangjun
dc.contributor.author
Martin, Gregory B.
dc.date.available
2019-09-24T22:10:34Z
dc.date.issued
2016-03
dc.identifier.citation
Worley, Jay N.; Pombo, Marina Alejandra; Zheng, Yi; Dunham, Diane M.; Myers, Christopher R.; et al.; A novel method of transcriptome interpretation reveals a quantitative suppressive effect on tomato immune signaling by two domains in a single pathogen effector protein; BioMed Central; BMC Genomics; 17; 3-2016; 1-13
dc.identifier.issn
1471-2164
dc.identifier.uri
http://hdl.handle.net/11336/84373
dc.description.abstract
Background: Effector proteins are translocated into host cells by plant-pathogens to undermine pattern-triggeredimmunity (PTI), the plant response to microbe-associated molecular patterns that interferes with the infection process. Individual effectors are found in variable repertoires where some constituents target the same pathways.The effector protein AvrPto from Pseudomonas syringae has a core domain (CD) and C-terminal domain (CTD) that each promotes bacterial growth and virulence in tomato. The individual contributions of each domain and whether they act redundantly is unknown.Results: We use RNA-Seq to elucidate the contribution of the CD and CTD to the suppression of PTI in tomato leaves 6 h after inoculation. Unexpectedly, each domain alters transcript levels of essentially the same genes but to a different degree. This difference, when quantified, reveals that although targeting the same host genes, the two domains act synergistically. AvrPto has a relatively greater effect on genes whose expression is suppressed during PTI, and the effect on these genes appears to be diminished by saturation.Conclusions: RNA-Seq profiles can be used to observe relative contributions of effector subdomains to PTI suppression. Our analysis shows the CD and CTD multiplicatively affect the same gene transcript levels with a greater relative impact on genes whose expression is suppressed during PTI. The higher degree of up-regulation versus down-regulation during PTI is plausibly an evolutionary adaptation against effectors that target immune signaling
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
BioMed Central
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Rna-Seq
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Pattern-Triggered Immunity
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Plant Immunity
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Type Iii Effectors
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Pseudomonas Syringae
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
A novel method of transcriptome interpretation reveals a quantitative suppressive effect on tomato immune signaling by two domains in a single pathogen effector protein
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-09-23T13:33:38Z
dc.journal.volume
17
dc.journal.pagination
1-13
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Worley, Jay N.. Boyce Thompson Institute for Plant Research; Estados Unidos
dc.description.fil
Fil: Pombo, Marina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina. Boyce Thompson Institute for Plant Research; Estados Unidos
dc.description.fil
Fil: Zheng, Yi. Boyce Thompson Institute for Plant Research; Estados Unidos
dc.description.fil
Fil: Dunham, Diane M.. Boyce Thompson Institute for Plant Research; Estados Unidos
dc.description.fil
Fil: Myers, Christopher R.. Cornell University; Estados Unidos
dc.description.fil
Fil: Fei, Zhangjun. Boyce Thompson Institute for Plant Research; Estados Unidos
dc.description.fil
Fil: Martin, Gregory B.. Boyce Thompson Institute for Plant Research; Estados Unidos. Cornell University; Estados Unidos
dc.journal.title
BMC Genomics
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/ 10.1186/s12864-016-2534-4
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-016-2534-4
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