Artículo

Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus

Echeverria, Maria GabrielaIcon ; Nosetto, Edgardo OmarIcon ; Etcheverrigaray, Maria Elisa
Fecha de publicación: 05/2000
Editorial: American Association of Veterinary Laboratory Diagnosticians
Revista: Journal of Veterinary Diagnostic Investigation
ISSN: 1040-6387
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:

Resumen

A blocking enzyme-linked immunosorbent assay (ELISA) using a urease conjugate (U-B-ELISA) was evaluated for screening sera for antibodies to pseudorabies virus under field conditions, A total of 764 serum samples were analyzed by U-B-ELISA. Of these, 264 were evaluated by both virus neutralization and U-B-ELISA, and the results were compared. U-B-ELISA showed 98.5% and 98.9% sensitivity and specificity, respectively. This test combines the sensitivity and specificity of the blocking ELISA format while allowing visual assessment of results.
Palabras clave: Elisa , Urease , Aujeszky
 
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/84164
DOI: http://dx.doi.org/10.1177/104063870001200313
URL: http://dx.doi.org/ journals.sagepub.com/doi/10.1177/104063870001200313
Colecciones
Articulos(IGEVET)
Articulos de INST.DE GENETICA VET ING FERNANDO NOEL DULOUT
Citación
Echeverria, Maria Gabriela; Nosetto, Edgardo Omar; Etcheverrigaray, Maria Elisa; Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus; American Association of Veterinary Laboratory Diagnosticians; Journal of Veterinary Diagnostic Investigation; 12; 3; 5-2000; 266-268
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