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dc.contributor.author
Virkel, Guillermo Leon
dc.contributor.author
Carletti, M.
dc.contributor.author
Cantiello, M.
dc.contributor.author
Della Donna, L.
dc.contributor.author
Gardini, G.
dc.contributor.author
Girolami, F.
dc.contributor.author
Nebbia, C.
dc.date.available
2019-09-18T20:51:50Z
dc.date.issued
2010-02
dc.identifier.citation
Virkel, Guillermo Leon; Carletti, M.; Cantiello, M.; Della Donna, L.; Gardini, G.; et al.; Characterization of xenobiotic metabolizing enzymes in bovine small intestinal mucosa; Wiley Blackwell Publishing, Inc; Journal of Veterinary Pharmacology and Therapeutics; 33; 3; 2-2010; 295-303
dc.identifier.issn
0140-7783
dc.identifier.uri
http://hdl.handle.net/11336/83897
dc.description.abstract
The intestinal mucosa plays a capital role in dictating the bioavailability of a large array of orally ingested drugs and toxicants. The activity and the expression of several xenobiotic metabolizing enzymes were measured in subcellular fractions from the duodenal mucosa of male veal calves and beef cattle displaying a functional rumen but differing in both age (about 8 months vs. 18 to 24 months) and dietary regimens (i.e., milk replacer plus hay and straw vs. corn and concentrated meal). Intestinal microsomes showed cytochrome P450 (CYP) 2B, 2C- and 3A-mediated activities and the presence of the corresponding immunorelated proteins, but no proof of CYP1A expression and/or functions could be provided. Intestinal microsomes were also active in performing reactions typically mediated by carboxylesterases (indophenylacetate hydrolysis), flavin-containing monooxygenases (methimazole S-oxidation), and uridindiphosphoglucuronyltransferases (1-naphthol glucuronidation), respectively. Cytosolic fractions displayed the glutathione S-transferase (GST)-dependent conjugation of 1-chloro-2,4-dinitrobenzene; besides, the GST-mediated conjugation of ethacrinic acid (GSTφ) or cumene hydroperoxide (GSTα) was matched by the presence of the corresponding immunorelated proteins. Conversely, despite the lack of measurable activity with 3,4-dichloronitrobenzene, a protein cross reacting with anti-rat GSTμ antibodies could be clearly detected. Although, as detected by densitometry, CYPs and GST isoenzymes tended to be more expressed in beef cattle than in veal calf preparations, there was a general poor correlation with the rate of the in vitro metabolism of the selected diagnostic probes.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Xenobiotic Metabolizing Enzymes
dc.subject
Intestinal Mucosa
dc.subject
Microsomes
dc.subject
Cattle
dc.subject.classification
Ciencias Veterinarias
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Ciencias Veterinarias
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CIENCIAS AGRÍCOLAS
dc.title
Characterization of xenobiotic metabolizing enzymes in bovine small intestinal mucosa
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-07-24T17:38:21Z
dc.journal.volume
33
dc.journal.number
3
dc.journal.pagination
295-303
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Virkel, Guillermo Leon. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
dc.description.fil
Fil: Carletti, M.. Universita degli Studi di Torino; Italia
dc.description.fil
Fil: Cantiello, M.. Universita degli Studi di Torino; Italia
dc.description.fil
Fil: Della Donna, L.. Universita degli Studi di Torino; Italia
dc.description.fil
Fil: Gardini, G.. Universita degli Studi di Torino; Italia
dc.description.fil
Fil: Girolami, F.. Universita degli Studi di Torino; Italia
dc.description.fil
Fil: Nebbia, C.. Universita degli Studi di Torino; Italia
dc.journal.title
Journal of Veterinary Pharmacology and Therapeutics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1365-2885.2009.01137.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2885.2009.01137.x
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