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Artículo

Evaluation of apoptosis markers in different cell lines infected with equine arteritis virus

Metz, German ErnestoIcon ; Abeyá, María MercedesIcon ; Serena, Maria SoledadIcon ; Panei, Carlos JavierIcon ; Echeverria, Maria GabrielaIcon
Fecha de publicación: 23/02/2019
Editorial: Informa Healthcare
Revista: Biotechnic and Histochemistry
ISSN: 1052-0295
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Veterinarias

Resumen

Equine arteritis virus (EAV) induces apoptosis in infected cells. Cell death caused by EAV has been studied mainly using three cell lines, BHK-21, RK-13 and Vero cells. The mechanism of apoptosis varies among cell lines and results cannot be correlated owing to differences in EAV strains used. We evaluated different markers for apoptosis in BHK-21, RK-13 and Vero cell lines using the Bucyrus EAV reference strain. Acridine orange/ethidium bromide staining revealed morphological changes in infected cells, while flow cytometry indicated the extent of apoptosis. We also observed DNA fragmentation, but the DNA ladder was detected at different times post-infection depending on the cell line, i.e., 48, 72 and 96 h post-infection in RK-13, Vero and BHK-21 cells, respectively. Measurement of viral titers obtained with each cell line indicated that apoptosis causes interference with viral replication and therefore decreased viral titers. As an unequivocal marker of apoptosis, we measured the expression of caspase-3 and caspases-8 and -9 as extrinsic and intrinsic markers of apoptosis pathways, respectively. Caspase-8 in BHK-21 cells was the only protease that was not detected at any of the times assayed. We found that Bucyrus EAV strain exhibited a distinctive apoptosis pathway depending on the cell line.
Palabras clave: Apoptosis , Caspases , Cell Lines , Dna Fragmentation , Equine Arteritis Virus , Flow Cytometry
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/83785
DOI: http://dx.doi.org/10.1080/10520295.2018.1521989
URL: https://www.tandfonline.com/doi/full/10.1080/10520295.2018.1521989
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Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Citación
Metz, German Ernesto; Abeyá, María Mercedes; Serena, Maria Soledad; Panei, Carlos Javier; Echeverria, Maria Gabriela; Evaluation of apoptosis markers in different cell lines infected with equine arteritis virus; Informa Healthcare; Biotechnic and Histochemistry; 94; 2; 23-2-2019; 115-125
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