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dc.contributor.author
Wisitruangsakul, Nattawadee
dc.contributor.author
Zebger, Ingo
dc.contributor.author
Ly, Khoa H.
dc.contributor.author
Murgida, Daniel Horacio
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dc.contributor.author
Ekgasit, Sanong
dc.contributor.author
Hildebrandt, Peter
dc.date.available
2019-09-16T21:38:20Z
dc.date.issued
2008-12
dc.identifier.citation
Wisitruangsakul, Nattawadee; Zebger, Ingo; Ly, Khoa H.; Murgida, Daniel Horacio; Ekgasit, Sanong; et al.; Redox-linked protein dynamics of cytochrome c probed by time-resolved surface enhanced infrared absorption spectroscopy; Royal Society of Chemistry; Physical Chemistry Chemical Physics; 10; 34; 12-2008; 5276-5286
dc.identifier.issn
1463-9076
dc.identifier.uri
http://hdl.handle.net/11336/83703
dc.description.abstract
Time-resolved surface enhanced infrared absorption (SEIRA) spectroscopy is employed to analyse the dynamics of the protein structural changes coupled to the electron transfer process of immobilised cytochrome c (Cyt-c). Upon electrostatic binding of Cyt-c to Au electrodes coated with self-assembled monolayers (SAMs) of carboxyl-terminated thiols, cyclic voltammetric measurements demonstrate a reversible redox process with a redox potential that is similar to that of Cyt-c in solution, and a non-exponential distance-dependence of the electron transfer rate as observed previously (D. H. Murgida and P. Hildebrandt, Chem. Soc. Rev. 2008, 37, 937). On the basis of characteristic redox-state-sensitive amide I bands, the protein structural changes triggered by the electron transfer are monitored by rapid scan and step scan SEIRA spectroscopy in combination with the potential jump technique. Whereas the temporal evolution of the conjugate bands at 1693 and 1673 cm -1 displays the same rate constants as electron transfer, the time-dependent changes of the 1660-cm-1 band are slower by about a factor of 2. The study demonstrates that time-resolved SEIRA spectroscopy provides further information about the dynamics and mechanism of interfacial processes of redox proteins, thereby complementing the results obtained from other surface-sensitive techniques. In comparison with previous surface enhanced resonance Raman spectroscopic findings, the present results are discussed in terms of the local electric field strengths at the Au/SAM/Cyt-c interface.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Royal Society of Chemistry
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Seira
dc.subject
Electron Transfer
dc.subject
Cytochrome
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Bioelectrochemistry
dc.subject.classification
Físico-Química, Ciencia de los Polímeros, Electroquímica
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dc.subject.classification
Ciencias Químicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Redox-linked protein dynamics of cytochrome c probed by time-resolved surface enhanced infrared absorption spectroscopy
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-06-11T18:14:18Z
dc.journal.volume
10
dc.journal.number
34
dc.journal.pagination
5276-5286
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Wisitruangsakul, Nattawadee. Technishe Universitat Berlin; Alemania
dc.description.fil
Fil: Zebger, Ingo. Technishe Universitat Berlin; Alemania
dc.description.fil
Fil: Ly, Khoa H.. Technishe Universitat Berlin; Alemania
dc.description.fil
Fil: Murgida, Daniel Horacio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
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Fil: Ekgasit, Sanong. Chulalongkorn University; Tailandia
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Fil: Hildebrandt, Peter. Technishe Universitat Berlin; Alemania
dc.journal.title
Physical Chemistry Chemical Physics
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1039/B806528D
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/pubs.rsc.org/en/content/articlelanding/2008/CP/b806528d
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