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Artículo

Bordetella parapertussis survives the innate interaction with human neutrophils by impairing bactericidal trafficking inside the cell through a lipid raft-dependent mechanism mediated by the lipopolysaccharide o antigen

Gorgojo, Juan PabloIcon ; Lamberti, Yanina AndreaIcon ; Valdez, Hugo AlbertoIcon ; Harvill, Eric T.; Rodriguez, Maria EugeniaIcon
Fecha de publicación: 12/2012
Editorial: American Society for Microbiology
Revista: Infection and Immunity
ISSN: 0019-9567
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biotecnología relacionada con la Salud

Resumen

Whooping cough is a reemerging disease caused by two closely related pathogens, Bordetella pertussis and Bordetella parapertussis. The incidence of B. parapertussis in whooping cough cases has been increasing since the introduction of acellular pertussis vaccines containing purified antigens that are common to both strains. Recently published results demonstrated that these vaccines do not protect against B. parapertussis due to the presence of the O antigen on the bacterial surface that impairs antibody access to shared antigens. We have investigated the effect of the lack of opsonization of B. parapertussis on the outcome of its interaction with human neutrophils (polymorphonuclear leukocytes [PMNs]). In the absence of opsonic antibodies, PMN interaction with B. parapertussis resulted in nonbactericidal trafficking upon phagocytosis. A high percentage of nonopsonized B. parapertussis was found in nonacidic lysosome marker (lysosome-associated membrane protein [LAMP])-negative phagosomes with access to the host cell-recycling pathway of external nutrients, allowing bacterial survival as determined by intracellular CFU counts. The lipopolysaccharide (LPS) O antigen was found to be involved in directing B. parapertussis to PMN lipid rafts, eventually determining the nonbactericidal fate inside the PMN. IgG opsonization of B. parapertussis drastically changed this interaction by not only inducing efficient PMN phagocytosis but also promoting PMN bacterial killing. These data provide new insights into the immune mechanisms of hosts against B. parapertussis and document the crucial importance of opsonic antibodies in immunity to this pathogen.
Palabras clave: Bordetella Parapertussis , O Antigen , Human Neutrophils , Immune Evasion
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/83629
URL: http://iai.asm.org/content/80/12/4309.abstract
DOI: http://dx.doi.org/10.1128/IAI.00662-12
Colecciones
Articulos(CINDEFI)
Articulos de CENT.DE INV EN FERMENTACIONES INDUSTRIALES (I)
Citación
Gorgojo, Juan Pablo; Lamberti, Yanina Andrea; Valdez, Hugo Alberto; Harvill, Eric T.; Rodriguez, Maria Eugenia; Bordetella parapertussis survives the innate interaction with human neutrophils by impairing bactericidal trafficking inside the cell through a lipid raft-dependent mechanism mediated by the lipopolysaccharide o antigen; American Society for Microbiology; Infection and Immunity; 80; 12; 12-2012; 4309-4316
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