Mostrar el registro sencillo del ítem

dc.contributor.author
Luzzani, Carlos Daniel
dc.contributor.author
Cardillo, Sabrina Beatriz
dc.contributor.author
Bermudez Moretti, Mariana
dc.contributor.author
Correa Garcia, Susana Raquel
dc.date.available
2019-09-10T18:13:01Z
dc.date.issued
2007-11
dc.identifier.citation
Luzzani, Carlos Daniel; Cardillo, Sabrina Beatriz; Bermudez Moretti, Mariana; Correa Garcia, Susana Raquel; New insights into the regulation of the Saccharomyces cerevisiae UGA54 gene: Two parallel pathways participate in carbon-regulated transcription; Society for General Microbiology; Microbiology-UK; 153; 11; 11-2007; 3677-3684
dc.identifier.issn
1350-0872
dc.identifier.uri
http://hdl.handle.net/11336/83262
dc.description.abstract
The Saccharomyces cerevisiae UGA4 gene, which encodes the γ-aminobutyric acid (GABA) and δ-aminolaevulinic acid (ALA) permease, is well known to be regulated by the nitrogen source. Its expression levels are low in the presence of a rich nitrogen source but are higher when a poor nitrogen source is used. In addition, GABA can induce UGA4 expression when cells are grown with proline but not when they are grown with ammonium. Although vast amounts of evidence have been gathered about UGA4 regulation by nitrogen, little is known about its regulation by the carbon source. Using glucose and acetate as rich and poor carbon source respectively, this work aimed to shed light on hitherto unclear aspects of the regulation of this gene. In poor nitrogen conditions, cells grown with acetate were found to have higher UGA4 basal expression levels than those grown with glucose, and did not show UGA4 induction in response to GABA. Analysis of the expression and subcellular localization of the transcription factors that regulate UGA4 as well as partial deletions and site-directed mutations of the UGA4 promoter region suggested that there are two parallel pathways that act in regulating this gene by the carbon source. Furthermore, the results demonstrate the existence of a new factor operating in UGA4 regulation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Society for General Microbiology
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Uga4
dc.subject
Yeast
dc.subject
Carbon Source
dc.subject.classification
Biología Celular, Microbiología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
New insights into the regulation of the Saccharomyces cerevisiae UGA54 gene: Two parallel pathways participate in carbon-regulated transcription
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-03-27T18:05:01Z
dc.journal.volume
153
dc.journal.number
11
dc.journal.pagination
3677-3684
dc.journal.pais
Reino Unido
dc.journal.ciudad
Reading
dc.description.fil
Fil: Luzzani, Carlos Daniel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Cardillo, Sabrina Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Bermudez Moretti, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Correa Garcia, Susana Raquel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Microbiology-UK
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1099/mic.0.2007/010231-0
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.2007/010231-0


Archivos asociados

Documento no disponible

Comunidades y colecciones

Mostrar el registro sencillo del ítem