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dc.contributor.author
Schumacher, Michael
dc.contributor.author
Guennoun, Rachida
dc.contributor.author
Mattern, Claudia
dc.contributor.author
Oudinet, Jean Paul
dc.contributor.author
Labombarda, Maria Florencia
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dc.contributor.author
de Nicola, Alejandro Federico
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dc.contributor.author
Liere, Phillippe
dc.date.available
2016-11-23T17:34:39Z
dc.date.issued
2015-11
dc.identifier.citation
Schumacher, Michael; Guennoun, Rachida; Mattern, Claudia; Oudinet, Jean Paul; Labombarda, Maria Florencia; et al.; Analytical challenges for measuring steroid responses to stress, neurodegeneration and injury in the central nervous system; Elsevier; Steroids; 103; 11-2015; 42-57
dc.identifier.issn
0039-128X
dc.identifier.uri
http://hdl.handle.net/11336/8301
dc.description.abstract
Levels of steroids in the adult central nervous system (CNS) show marked changes in response to stress, degenerative disorders and injury. However, their analysis in complex matrices such as fatty brain and spinal cord tissues, and even in plasma, requires accurate and precise analytical methods. Radioimmunoassays (RIA) and enzyme-linked immunosorbent assays, even with prepurification steps, do not provide sufficient specificity, and they are at the origin of many inconsistent results in the literature. The analysis of steroids by mass spectrometric methods has become the gold standard for accurate and sensitive steroid analysis. However, these technologies involve multiple purification steps prone to errors, and they only provide accurate reference values when combined with careful sample workup. In addition, the interpretation of changes in CNS steroid levels is not an easy task because of their multiple sources: the endocrine glands and the local synthesis by neural cells. In the CNS, decreased steroid levels may reflect alterations of their biosynthesis, as observed in the case of chronic stress, post-traumatic stress disorders or depressive episodes. In such cases, return to normalization by administering exogenous hormones or by stimulating their endogenous production may have beneficial effects. On the other hand, increases in CNS steroids in response to acute stress, degenerative processes or injury may be part of endogenous protective or rescue programs, contributing to the resistance of neural cells to stress and insults. The aim of this review is to encourage a more critical reading of the literature reporting steroid measures, and to draw attention to the absolute need for well-validated methods. We discuss reported findings concerning changing steroid levels in the nervous system by insisting on methodological issues. An important message is that even recent mass spectrometric methods have their limits, and they only become reliable tools if combined with careful sample preparation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Alzheimer’ S Disease
dc.subject
Multiple Sclerosis
dc.subject
Brain Injury
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Neurosteroid
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Stress
dc.subject.classification
Neurociencias
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Analytical challenges for measuring steroid responses to stress, neurodegeneration and injury in the central nervous system
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-10-04T19:27:01Z
dc.identifier.eissn
1878-5867
dc.journal.volume
103
dc.journal.pagination
42-57
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Schumacher, Michael. Inserm; Francia. Universite Paris Sud; Francia
dc.description.fil
Fil: Guennoun, Rachida. Inserm; Francia. Universite Paris Sud; Francia
dc.description.fil
Fil: Mattern, Claudia. M et P Pharma AG; Suiza
dc.description.fil
Fil: Oudinet, Jean Paul. Inserm; Francia. Universite Paris Sud; Francia
dc.description.fil
Fil: Labombarda, Maria Florencia. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: de Nicola, Alejandro Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
dc.description.fil
Fil: Liere, Phillippe. Inserm; Francia
dc.journal.title
Steroids
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.steroids.2015.08.013
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/ttp://www.sciencedirect.com/science/article/pii/S0039128X15002275
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