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dc.contributor.author
Rodriguez, Marcela Cecilia  
dc.contributor.author
Rivas, Gustavo Adolfo  
dc.date.available
2019-09-03T17:42:20Z  
dc.date.issued
2009-04  
dc.identifier.citation
Rodriguez, Marcela Cecilia; Rivas, Gustavo Adolfo; Label-free electrochemical aptasensor for the detection of lysozyme; Elsevier Science; Talanta; 78; 1; 4-2009; 212-216  
dc.identifier.issn
0039-9140  
dc.identifier.uri
http://hdl.handle.net/11336/82798  
dc.description.abstract
This work reports the advantages of a label free electrochemical aptasensor for the detection of lysozyme. The biorecognition platform was obtained by the adsorption of the aptamer on the surface of a carbon paste electrode (CPE) previously blocked with mouse immunoglobulin under controlled-potential conditions. The recognition event was detected from the decrease in the guanine and adenine electro-oxidation signals produced as a consequence of the molecular interaction between the aptamer and lysozyme. The biosensing platform demonstrated to be highly selective even in the presence of large excess (9-fold) of bovine serum albumin, cytochrome C and myoglobin. The reproducibility for 10 repetitive determinations of 10.0 mg L-1 lysozyme solution was 5.1% and 6.8% for guanine and adenine electro-oxidation signals, respectively. The detection limits of the aptasensor were 36.0 nmol L-1 (if considering guanine signal) and 18.0 nmol L-1 (if taking adenine oxidation current). This new sensing approach represents an interesting and promising alternative for the electrochemical quantification of lysozyme.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Aptamer  
dc.subject
Biosensor  
dc.subject
Carbon Paste Electrode  
dc.subject
Electrochemical Aptasensor  
dc.subject
Lysozyme  
dc.subject
Protein Detection  
dc.subject.classification
Química Analítica  
dc.subject.classification
Ciencias Químicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Label-free electrochemical aptasensor for the detection of lysozyme  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-05-06T13:48:13Z  
dc.journal.volume
78  
dc.journal.number
1  
dc.journal.pagination
212-216  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Rodriguez, Marcela Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina  
dc.description.fil
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina  
dc.journal.title
Talanta  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S003991400800806  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.talanta.2008.11.002