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dc.contributor.author
Cazorla, Silvia Ines
dc.contributor.author
Maldonado Galdeano, María Carolina
dc.contributor.author
Weill, Ricardo
dc.contributor.author
De Paula, Juan
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Perdigon, Gabriela del Valle
dc.date.available
2019-08-30T18:37:21Z
dc.date.issued
2018-04-16
dc.identifier.citation
Cazorla, Silvia Ines; Maldonado Galdeano, María Carolina; Weill, Ricardo; De Paula, Juan; Perdigon, Gabriela del Valle; Oral administration of probiotics increases paneth cells and intestinal antimicrobial activity; Frontiers Media SA; Frontiers in Microbiology; 9; 736; 16-4-2018; 1-14
dc.identifier.issn
1664-302X
dc.identifier.uri
http://hdl.handle.net/11336/82629
dc.description.abstract
The huge amount of intestinal bacteria represents a continuing threat to the intestinal barrier. To meet this challenge, gut epithelial cells produce antimicrobial peptides (AMP) that act at the forefront of innate immunity. We explore whether this antimicrobial activity and Paneth cells, the main intestinal cell responsible of AMP production, are influenced by probiotics administration, to avoid the imbalance of intestinal microbiota and preserve intestinal barrier. Administration of Lactobacillus casei CRL 431 (Lc 431) and L. paracasei CNCM I-1518 (Lp 1518) to 42 days old mice, increases the number of Paneth cells on small intestine, and the antimicrobial activity against the pathogens Staphylococcus aureus and Salmonella Typhimurium in the intestinal fluids. Specifically, strong damage of the bacterial cell with leakage of cytoplasmic content, and cellular fragmentation were observed in S. Typhimurium and S. aureus. Even more important, probiotics increase the antimicrobial activity of the intestinal fluids at the different ages, from weaning (21 days old) to old age (180 days old). Intestinal antimicrobial activity stimulated by oral probiotics, do not influence significantly the composition of total anaerobic bacteria, lactobacilli and enterobacteria in the large intestine, at any age analyzed. This result, together with the antimicrobial activity observed against the same probiotic bacteria; endorse the regular consumption of probiotics without adverse effect on the intestinal homeostasis in healthy individuals. We demonstrate that oral probiotics increase intestinal antimicrobial activity and Paneth cells in order to strengthen epithelial barrier against pathogens. This effect would be another important mechanism by which probiotics protect the host mainly against infectious diseases.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media SA
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Antimicrobial Activity
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Intestinal Fluids
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Intestinal Microbiota
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Paneth Cells
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Probiotics
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Oral administration of probiotics increases paneth cells and intestinal antimicrobial activity
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-08-20T14:14:30Z
dc.identifier.eissn
1664-302X
dc.journal.volume
9
dc.journal.number
736
dc.journal.pagination
1-14
dc.journal.pais
Suiza
dc.journal.ciudad
Lausanne
dc.description.fil
Fil: Cazorla, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
dc.description.fil
Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
dc.description.fil
Fil: Weill, Ricardo. DANONE. Departamento de Investigación y Desarrollo; Argentina
dc.description.fil
Fil: De Paula, Juan. Hospital Italiano. Servicio de Gastroenterología; Argentina
dc.description.fil
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
dc.journal.title
Frontiers in Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fmicb.2018.00736/full
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.3389/fmicb.2018.00736
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