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dc.contributor.author
Gianni de Carvalho, Katia  
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Bambirra, Felipe H. S.  
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Nicoli, Jacques R.  
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Oliveira, Jamil S.  
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Santos, Alexandre M. C.  
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Bemquerer, Marcelo P.  
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Miranda, Antonio  
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Franco, Bernadette D. G. M.  
dc.date.available
2019-08-28T12:38:57Z  
dc.date.issued
2018-03  
dc.identifier.citation
Gianni de Carvalho, Katia; Bambirra, Felipe H. S.; Nicoli, Jacques R.; Oliveira, Jamil S.; Santos, Alexandre M. C.; et al.; Characterization of multiple antilisterial peptides produced by sakacin P-producing Lactobacillus sakei subsp. sakei 2a; Springer; Archives of Microbiology; 200; 4; 3-2018; 635–644  
dc.identifier.issn
0302-8933  
dc.identifier.uri
http://hdl.handle.net/11336/82332  
dc.description.abstract
Antimicrobial compounds produced by lactic acid bacteria can be explored as natural food biopreservatives. In a previous report, the main antimicrobial compounds produced by the Brazilian meat isolate Lactobacillus sakei subsp. sakei 2a, i.e., bacteriocin sakacin P and two ribosomal peptides (P2 and P3) active against Listeria monocytogenes, were described. In this study, we report the spectrum of activity, molecular mass, structural identity and mechanism of action of additional six antilisterial peptides produced by Lb. sakei 2a, detected in a 24 h-culture in MRS broth submitted to acid treatment (pH 1.5) and proper fractionation and purification steps for obtention of free and cell-bound proteins. The six peptides presented similarity to different ribosomal proteins of Lb. sakei subsp sakei 23K and the molecular masses varied from 4.6 to 11.0 kDa. All peptides were capable to increase the efflux of ATP and decrease the membrane potential in Listeria monocytogenes. The activity of a pool of the obtained antilisterial compounds [enriched active fraction (EAF)] against Listeria monocytogenes in a food model (meat gravy) during refrigerated storage (4 °C) for 10 days was also tested and results indicated that the populations of L. monocytogenes in the food model containing the acid extract remained lower than those at time 0-day, evidencing that the acid extract of a culture of Lb. sakei 2a is a good technological alternative for the control of growth of L. monocytogenes in foods.  
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application/pdf  
dc.language.iso
eng  
dc.publisher
Springer  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Biopreservation  
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Lactobacillus Sakei Subsp. Sakei 2a  
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Listeria Monocytogenes  
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Meat Gravy  
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Multiple Antimicrobial Peptides  
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Biología Celular, Microbiología  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Characterization of multiple antilisterial peptides produced by sakacin P-producing Lactobacillus sakei subsp. sakei 2a  
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info:eu-repo/semantics/article  
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info:ar-repo/semantics/artículo  
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info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-08-27T13:27:50Z  
dc.journal.volume
200  
dc.journal.number
4  
dc.journal.pagination
635–644  
dc.journal.pais
Alemania  
dc.journal.ciudad
Heidelberg  
dc.description.fil
Fil: Gianni de Carvalho, Katia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
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Fil: Bambirra, Felipe H. S.. Universidade Federal de Minas Gerais; Brasil  
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Fil: Nicoli, Jacques R.. Universidade Federal de Minas Gerais; Brasil  
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Fil: Oliveira, Jamil S.. Universidade Federal de Minas Gerais; Brasil  
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Fil: Santos, Alexandre M. C.. Universidade Federal de Minas Gerais; Brasil. Universidade Federal do Espírito Santo; Brasil  
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Fil: Bemquerer, Marcelo P.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil  
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Fil: Miranda, Antonio. Universidade Federal de Sao Paulo;  
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Fil: Franco, Bernadette D. G. M.. Universidade de Sao Paulo; Brasil  
dc.journal.title
Archives of Microbiology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1007/s00203-018-1477-3  
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info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00203-018-1477-3