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Artículo

Development and assessment of an improved recombinant multiepitope antigen-based immunoassay to diagnose chronic Chagas disease

Peverengo, Luz MaríaIcon ; Garcia, Valeria SoledadIcon ; Rodeles Antonelli, Luz MaríaIcon ; Mendicino, Diego; Vicco, Miguel HernánIcon ; Lagier, Claudia MarinaIcon ; González, Verónica Doris GuadalupeIcon ; Gugliotta, Luis MarcelinoIcon ; Marcipar, Iván SergioIcon
Fecha de publicación: 10/2018
Editorial: Cambridge University Press
Revista: Parasitology
ISSN: 0031-1820
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biotecnología relacionada con la Salud

Resumen

The use of chimeric molecules fusing several antigenic determinants is a promising strategy for the development of low-cost, standardized and reliable kits to determine specific antibodies. In this study, we designed and assessed a novel recombinant chimera that complements the performance of our previously developed chimera, CP1 [FRA and SAPA antigens (Ags)], to diagnose chronic Chagas disease. The new chimeric protein, named CP3, is composed of MAP, TcD and TSSAII/V/VI antigenic determinants. We compared the performance of both chimeric Ags using a panel of 67 Trypanosoma cruzi-reactive sera and 67 non-reactive ones. The sensitivity of CP3 vs CP1 was 100 and 90.2%, and specificity was 92.5 and 100%, respectively. The mixture of CP1 + CP3 achieved 100% of sensitivity and specificity. More importantly, an additional subset of 17 sera from patients with discordant results of conventional serological methods was analysed; the CP1 + CP3 mixture allowed us to accurately classify 14 of them with respect to IIF, the usual technique used in most of the reference centres. These results show an improved performance of the CP1 + CP3 mixture in comparison with enzyme-linked immunosorbent assay and indirect haemagglutination commercial assays.
Palabras clave: Chagas Disease , Chimera , Diagnostic , Multiepitope , Recombinant Protein , Trypanosoma Cruzi
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/82277
DOI: https://doi.org/10.1017/S0031182018000458
URL: https://www.cambridge.org/core/journals/parasitology/article/development-and-ass
Colecciones
Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos(INTEC)
Articulos de INST.DE DES.TECNOL.PARA LA IND.QUIMICA (I)
Articulos(IQUIR)
Articulos de INST.DE QUIMICA ROSARIO
Citación
Peverengo, Luz María; Garcia, Valeria Soledad; Rodeles Antonelli, Luz María; Mendicino, Diego; Vicco, Miguel Hernán; et al.; Development and assessment of an improved recombinant multiepitope antigen-based immunoassay to diagnose chronic Chagas disease; Cambridge University Press; Parasitology; 145; 12; 10-2018; 1594-1599
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