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dc.contributor.author
Romero, Cintia Mariana  
dc.contributor.author
Spuches, Florencia Cecilia  
dc.contributor.author
Morales, Andrés Hernán  
dc.contributor.author
Perotti, Nora Ines  
dc.contributor.author
Navarro, Maria Carolina  
dc.contributor.author
Gómez, María Inés  
dc.date.available
2019-08-27T15:03:07Z  
dc.date.issued
2018-12  
dc.identifier.citation
Romero, Cintia Mariana; Spuches, Florencia Cecilia; Morales, Andrés Hernán; Perotti, Nora Ines; Navarro, Maria Carolina; et al.; Design and characterization of immobilized biocatalyst with lipase activity onto magnetic magnesium spinel nanoparticles: A novel platform for biocatalysis; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 172; 12; 12-2018; 699-707  
dc.identifier.issn
0927-7765  
dc.identifier.uri
http://hdl.handle.net/11336/82219  
dc.description.abstract
Lipases (EC 3.1.1.3) are very used industrial enzymes but presents drawbacks such as lack of stability, and poor recyclability. Most of these obstacles can be solved by lipase immobilization. The objective of this work was evaluated to magnetic magnesium spinel nanoparticles as support for lipase immobilization by covalent bound. The techniques used for nanoparticles synthesis presented advantages in the size selection of the nanoparticles obtained (60?100 nm). The immobilization of Candida rugosa lipase (CRL) was optimized. The optimal conditions were determined to be pH 3.7, enzyme concentration of 1.1 mg/mL at 4 °C and an ionic strength of 100 mM. The CRL@MgFe 2 O 4 activity obtained was 3.2 times over the starting conditions (4.03 U/mL). The immobilization of the lipase on Fe 3 O 4 was evaluated and compared. The activity of the CRL@MgFe 2 O 4 was 61% higher than CRL@Fe 3 O 4 and 22% higher than free enzyme. CRL@MgFe 2 O 4 improved the lipase stability at alkaline pH, hydrophilic solvent and high temperatures. The thermogravimetric analysis showed that this new biocatalyst was more stable compared to the free enzyme. Additionally, the immobilized lipase was recycled by magnetic force and used in ten catalysis cycles. The performance of the recycle was improved using butanol or Triton X 100 during washing. Finally, CRL@FeMg 2 O 4 showed hydrolysis and synthesis activity. Thus, CRL@FeMg 2 O 4 as a novel biocatalyst generation presents interesting properties for industrial applications.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Immobilization  
dc.subject
Lipase  
dc.subject
Magnetic Magnesium Spinel Nanoparticle  
dc.subject
Novel Biocatalyst  
dc.subject.classification
Físico-Química, Ciencia de los Polímeros, Electroquímica  
dc.subject.classification
Ciencias Químicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Design and characterization of immobilized biocatalyst with lipase activity onto magnetic magnesium spinel nanoparticles: A novel platform for biocatalysis  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-08-27T13:34:42Z  
dc.journal.volume
172  
dc.journal.number
12  
dc.journal.pagination
699-707  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Romero, Cintia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
dc.description.fil
Fil: Spuches, Florencia Cecilia. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
dc.description.fil
Fil: Morales, Andrés Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
dc.description.fil
Fil: Perotti, Nora Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
dc.description.fil
Fil: Navarro, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
dc.description.fil
Fil: Gómez, María Inés. Universidad Nacional de Tucumán; Argentina  
dc.journal.title
Colloids and Surfaces B: Biointerfaces  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.journals.elsevier.com/colloids-and-surfaces-b-biointerfaces  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.colsurfb.2018.08.071