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dc.contributor.author
Oviedo, Maria Silvina
dc.contributor.author
Sturm, María Elena
dc.contributor.author
Reynoso, Maria Marta
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dc.contributor.author
Chulze, Sofia Noemi
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dc.contributor.author
Ramirez, Maria Laura
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dc.date.available
2015-06-18T17:52:43Z
dc.date.issued
2013-10
dc.identifier.citation
Oviedo, Maria Silvina; Sturm, María Elena; Reynoso, Maria Marta ; Chulze, Sofia Noemi; Ramirez, Maria Laura; Toxigenic profile and AFLP variability of Alternaria alternata and Alternaria infectoria occurring on wheat; Soc Brasileira Microbiologia; Brazilian Journal Of Microbiology; 44; 10-2013; 247-455
dc.identifier.issn
1517-8382
dc.identifier.uri
http://hdl.handle.net/11336/821
dc.description.abstract
Abstract The objectives of this study were to evaluate the ability to produce alternariol (AOH), alternariol monomethyl ether (AME) and tenuazonic acid (TA) by A. alternata and A. infectoria strains recovered from wheat kernels obtained from one of the main production area in Argentina; to confirm using AFLPs molecular markers the identity of the isolates up to species level, and to evaluate the intra and inter-specific genetic diversity of these two Alternaria species. Among all the Alternaria strains tested (254), 84% of them were able to produce mycotoxins. The most frequent profile of toxin production found was the co-production of AOH and AME in both species tested. TA was only produced by strains of A. alternata. Amplified fragment polymorphism (AFLPs) analysis was applied to a set of 89 isolates of Alternaria spp (40 were A. infectoria and 49 were A. alternata) in order to confirm the morphological identification. The results showed that AFLPs are powerful diagnostic tool for differentiating between A. alternata and A. infectoria. Indeed, in the current study the outgroup strains, A. tenuissima was consistently classified. Characteristic polymorphic bands separated these two species regardless of the primer combination used. Related to intraspecific variability, A. alternata and A. infectoria isolates evaluated seemed to form and homogeneous group with a high degree of similarity among the isolates within each species. However, there was more scoreable polymorphism within A. alternata than within A. infectoria isolates. There was a concordance between morphological identification and separation up to species level using molecular markers. Clear polymorphism both within and between species showed that AFLP can be used to asses genetic variation in A. alternata and A. infectoria. The most important finding of the present study was the report on AOH and AME production by A. infectoria strains isolated from wheat kernels in Argentina on a semisynthetic media for the first time. Also, specific bands for A. alternata and A. infectoria have been identified; these may be useful for the design of specific PCR primers in order to differentiate these species and to detect them in cereals.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Soc Brasileira Microbiologia
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.source
www.scielo.br/bjm
dc.subject
Alternaria Alternata
dc.subject
Alternaria Infectoria
dc.subject
Alternariol, Alternariol Monomethyl Ether, Tenuazonic Acid
dc.subject
Aflps
dc.subject
Wheat
dc.subject
Genetic Variation
dc.subject.classification
Ciencias Naturales y Exactas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
Micología
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dc.title
Toxigenic profile and AFLP variability of Alternaria alternata and Alternaria infectoria occurring on wheat
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.identifier.eissn
1678-4405
dc.journal.volume
44
dc.journal.pagination
247-455
dc.journal.pais
Brasil
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dc.description.fil
Fil: Oviedo, Maria Silvina. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Cat.de Micología; Argentina;
dc.description.fil
Fil: Sturm, María Elena. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia; Argentina;
dc.description.fil
Fil: Reynoso, Maria Marta. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia; Argentina;
dc.description.fil
Fil: Chulze, Sofia Noemi. Universidad Nacional de Cordoba. Facultad de Cs.quimicas. Departamento de Bioquimica Clinica. Cat.de Inmunologia; Argentina;
dc.description.fil
Fil: Ramirez, Maria Laura. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia; Argentina;
dc.journal.title
Brazilian Journal Of Microbiology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833142/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1590/S1517-83822013000200017
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