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dc.contributor.author
Castro, Gerardo Daniel
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dc.contributor.author
Costantini, Martin Hernan
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dc.contributor.author
Castro, Jose Alberto
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dc.date.available
2019-08-26T18:22:53Z
dc.date.issued
2009-04
dc.identifier.citation
Castro, Gerardo Daniel; Costantini, Martin Hernan; Castro, Jose Alberto; Rat ventral prostate xanthine oxidaseg-mediated metabolism of acetaldehyde to acetyl radical; Sage Publications Ltd; Human and Experimental Toxicoloxy; 28; 4; 4-2009; 203-208
dc.identifier.issn
0960-3271
dc.identifier.uri
http://hdl.handle.net/11336/82112
dc.description.abstract
Alcohol drinking is known to lead to deleterious effects on prostate epithelial cells from humans and experimental animals. The understanding of the mechanisms underlying these effects is relevant to intraprostatic ethanol treatment of benign prostatic hyperplasia and to shed some light into the conflictive results linking alcohol consumption to prostate cancer. In previous studies, we provided evidence about the presence in the rat ventral prostate of cytosolic and microsomal metabolic pathways of ethanol to acetaldehyde and 1-hydroxyethyl radical and about the low levels of alcohol dehydrogenase and aldehyde dehydrogenase. Acetaldehyde accumulation in prostate tissue and oxidative stress promotion were also observed. In this study, we report that in the ventral prostate cytosolic fraction, xanthine oxidoreductase is able to metabolize acetaldehyde to acetyl radical. The identification of the acetyl was performed by GC-MS of the silylated acetyl-PBN adduct. Reference adduct was generated chemically. Formation of acetyl was also observed using pure xanthine oxidase. The generation of acetyl by the prostate cytosol was inhibited by allopurinol, oxypurinol, diphenyleneiodonium chloride, folate, and ellagic acid. Results suggest that metabolism of ethanol to acetaldehyde and to 1-hydroxyethyl and acetyl radicals could be involved in the deleterious effects of alcohol drinking on prostate epithelial cells.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Sage Publications Ltd
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Acetaldehyde
dc.subject
Acetyl
dc.subject
Alcohol
dc.subject
Free Radicals
dc.subject
Prostate
dc.subject
Xanthine Oxidase
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Rat ventral prostate xanthine oxidaseg-mediated metabolism of acetaldehyde to acetyl radical
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-08-22T13:21:33Z
dc.journal.volume
28
dc.journal.number
4
dc.journal.pagination
203-208
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Castro, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
dc.description.fil
Fil: Costantini, Martin Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
dc.description.fil
Fil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
dc.journal.title
Human and Experimental Toxicoloxy
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/abs/10.1177/0960327109105406
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1177/0960327109105406
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