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dc.contributor.author
Milanesi, Lorena Magdalena
dc.contributor.author
Boland, Ricardo Leopoldo
dc.date.available
2019-08-21T17:29:49Z
dc.date.issued
2006-07-26
dc.identifier.citation
Milanesi, Lorena Magdalena; Boland, Ricardo Leopoldo; Presence of vitamin D3 receptor (VDR)-like proteins in solanum glaucophyllum; Wiley Blackwell Publishing, Inc; Physiologia Plantarum; 128; 2; 26-7-2006; 341-350
dc.identifier.issn
0031-9317
dc.identifier.uri
http://hdl.handle.net/11336/81929
dc.description.abstract
The detection of 1α,25(OH)2‐vitamin D3 [1α,25(OH)2D3] in Solanum glaucophyllum and other plant species has been reported. Little is known about the mode of action of 1α,25(OH)2D3 in plants. The steroid acts in animal systems through binding to a specific receptor (VDR). In the present work, competition assays allowed the detection of binding sites for [3H]1α,25(OH)2D3 in cultured S. glaucophyllum cells. The binding of [3H]1α,25(OH)2D3 to total protein from calli was a saturable process with respect to the ligand concentration (Kd= 3.49 ± 0.75 nM; Bmax= 255.7 ± 18.6 fmol/mg protein). Western blots employing antibodies directed against the avian VDR revealed three immunoreactive bands of approximately 72, 58 and 43 kDa in different plant organs, cells and callus cultures. The immunoreactive proteins were tyrosine phosphorylated, and the signal detected was increased using hypertonic buffers for protein extraction, suggesting that these proteins are associated with subcellular structures. When the callus cells were subjected to homogenization and differential centrifugation, a substantial proportion of the immunolabeling was localized in the nuclear and mitochondrial fractions. Ligand blot assays revealed the ability of the reactive bands to bind [3H]1α,25(OH)2D3. Immunocytochemistry detection using Oregon Green‐conjugated secondary antibodies showed immunofluorescent staining mainly in the nucleus, and in the nucleus, cytoplasmic corpuscles and outer cell membrane, for the monoclonal and polyclonal antibodies, respectively. [3H]Thymidine incorporation assays and immunoblot analysis of mitogen‐activated protein kinase (MAPK) indicated that 1α,25(OH)2D3 and cell lipid extracts stimulate DNA synthesis but do not activate MAPKs with TEY domains in S. glaucophyllum cells. These data demonstrate that S. glaucophyllum cells, which produce 1α,25(OH)2D3, also express a cognate binding protein(s) and are able to respond to 1α,25(OH)2D3. The exact functional role of the VDR‐like protein (s) remains to be established.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Presence of vitamin D3 receptor (VDR)-like proteins in solanum glaucophyllum
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-08-16T15:49:38Z
dc.identifier.eissn
1399-3054
dc.journal.volume
128
dc.journal.number
2
dc.journal.pagination
341-350
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Milanesi, Lorena Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.description.fil
Fil: Boland, Ricardo Leopoldo. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina
dc.journal.title
Physiologia Plantarum
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1399-3054.2006.00742.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1399-3054.2006.00742.x
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