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dc.contributor.author Regueira, Mariana
dc.contributor.author Artagaveytia, Silvana Lucía
dc.contributor.author Galardo, Maria Noel Lujan
dc.contributor.author Pellizzari, Eliana Herminia
dc.contributor.author Cigorraga, Selva Beatriz
dc.contributor.author Meroni, Silvina Beatriz
dc.contributor.author Riera, Maria Fernanda
dc.date.available 2016-11-14T17:37:21Z
dc.date.issued 2015-08
dc.identifier.citation Regueira, Mariana; Artagaveytia, Silvana Lucía; Galardo, Maria Noel Lujan; Pellizzari, Eliana Herminia; Cigorraga, Selva Beatriz; et al.; Novel molecular mechanisms involved in hormonal regulation of lactate production in Sertoli cells; Bioscientifica; Reproduction; 150; 8-2015; 311-321
dc.identifier.issn 1470-1626
dc.identifier.uri http://hdl.handle.net/11336/8179
dc.description.abstract The aim of the study was to analyze molecular mechanisms involved in follicle-stimulating hormone (FSH) and basic Fibroblast Growth Factor (bFGF) regulation of lactate production in rat Sertoli cells. The regulation of pyruvate availability, which is converted to lactate, could be a mechanism utilized by hormones to ensure lactate supply to germ cells. On one hand, the regulation of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase (PFKFB) expression could result in increased glycolysis, while an increase in pyruvate availability may also result from a lower conversion to acetyl-CoA by negative regulation of pyruvate dehydrogenase complex (PDC) activity by phosphorylation. Sertoli cells cultures obtained from 20-day-old rats were used. Stimulation of the cultures with FSH or bFGF showed that FSH increases Pfkfb1 and Pfkfb3 expression while bFGF increases Pfkfb1 mRNA levels. Additionally, we observed that FSH-stimulated lactate production was inhibited in the presence of a PFKFB3 inhibitor, revealing the physiological relevance of this mechanism. As for the regulation of PDC, analysis of pyruvate dehydrogenase kinase (Pdk) expression showed that FSH increases Pdk3 and decreases Pdk4 mRNA levels while bFGF increases the expression of all Pdks. In addition, we showed that bFGF increases phosphorylated PDC levels and that bFGF stimulated lactate production is partially inhibited in the presence of a PDK inhibitor. Altogether, these results add new information regarding novel molecular mechanisms involved in hormonal regulation of lactate production in Sertoli cells. Considering that lactate is essential for the production of energy in spermatocytes and spermatids, these mechanisms might be relevant in maintaining spermatogenesis and male fertility.
dc.format application/pdf
dc.language.iso eng
dc.publisher Bioscientifica
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject SERTOLI CELL
dc.subject LACTATE
dc.subject PFKFB
dc.subject PDC
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Novel molecular mechanisms involved in hormonal regulation of lactate production in Sertoli cells
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2016-11-11T16:52:26Z
dc.journal.volume 150
dc.journal.pagination 311-321
dc.journal.pais Reino Unido
dc.journal.ciudad Bristol
dc.description.fil Fil: Regueira, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.description.fil Fil: Artagaveytia, Silvana Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.description.fil Fil: Galardo, Maria Noel Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.description.fil Fil: Pellizzari, Eliana Herminia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.description.fil Fil: Cigorraga, Selva Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.description.fil Fil: Meroni, Silvina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.description.fil Fil: Riera, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.journal.title Reproduction
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/150/4/311.long
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1530/REP-15-0093


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    Articulos de CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"

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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)