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dc.contributor.author
Regueira, Mariana
dc.contributor.author
Artagaveytia, Silvana Lucía
dc.contributor.author
Galardo, Maria Noel Lujan
dc.contributor.author
Pellizzari, Eliana Herminia
dc.contributor.author
Cigorraga, Selva Beatriz
dc.contributor.author
Meroni, Silvina Beatriz
dc.contributor.author
Riera, Maria Fernanda
dc.date.available
2016-11-14T17:37:21Z
dc.date.issued
2015-08
dc.identifier.citation
Regueira, Mariana; Artagaveytia, Silvana Lucía; Galardo, Maria Noel Lujan; Pellizzari, Eliana Herminia; Cigorraga, Selva Beatriz; et al.; Novel molecular mechanisms involved in hormonal regulation of lactate production in Sertoli cells; Bioscientifica; Reproduction; 150; 8-2015; 311-321
dc.identifier.issn
1470-1626
dc.identifier.uri
http://hdl.handle.net/11336/8179
dc.description.abstract
The aim of the study was to analyze molecular mechanisms involved in follicle-stimulating hormone (FSH) and basic Fibroblast Growth Factor (bFGF) regulation of lactate production in rat Sertoli cells. The regulation of pyruvate availability, which is converted to lactate, could be a mechanism utilized by hormones to ensure lactate supply to germ cells. On one hand, the regulation of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase (PFKFB) expression could result in increased glycolysis, while an increase in pyruvate availability may also result from a lower conversion to acetyl-CoA by negative regulation of pyruvate dehydrogenase complex (PDC) activity by phosphorylation. Sertoli cells cultures obtained from 20-day-old rats were used. Stimulation of the cultures with FSH or bFGF showed that FSH increases Pfkfb1 and Pfkfb3 expression while bFGF increases Pfkfb1 mRNA levels. Additionally, we observed that FSH-stimulated lactate production was inhibited in the presence of a PFKFB3 inhibitor, revealing the physiological relevance of this mechanism. As for the regulation of PDC, analysis of pyruvate dehydrogenase kinase (Pdk) expression showed that FSH increases Pdk3 and decreases Pdk4 mRNA levels while bFGF increases the expression of all Pdks. In addition, we showed that bFGF increases phosphorylated PDC levels and that bFGF stimulated lactate production is partially inhibited in the presence of a PDK inhibitor. Altogether, these results add new information regarding novel molecular mechanisms involved in hormonal regulation of lactate production in Sertoli cells. Considering that lactate is essential for the production of energy in spermatocytes and spermatids, these mechanisms might be relevant in maintaining spermatogenesis and male fertility.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Bioscientifica
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Sertoli Cell
dc.subject
Lactate
dc.subject
Pfkfb
dc.subject
Pdc
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Novel molecular mechanisms involved in hormonal regulation of lactate production in Sertoli cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-11-11T16:52:26Z
dc.journal.volume
150
dc.journal.pagination
311-321
dc.journal.pais
Reino Unido
dc.journal.ciudad
Bristol
dc.description.fil
Fil: Regueira, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.description.fil
Fil: Artagaveytia, Silvana Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.description.fil
Fil: Galardo, Maria Noel Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.description.fil
Fil: Pellizzari, Eliana Herminia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.description.fil
Fil: Cigorraga, Selva Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.description.fil
Fil: Meroni, Silvina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.description.fil
Fil: Riera, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.journal.title
Reproduction
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/150/4/311.long
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1530/REP-15-0093


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