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dc.contributor.author
Acquaviva, Agustín  
dc.contributor.author
Jones, Andrew  
dc.contributor.author
Dennis, Gary R.  
dc.contributor.author
Castells, Cecilia Beatriz Marta  
dc.contributor.author
Shalliker, R. Andrew  
dc.contributor.author
Soliven, Arianne  
dc.date.available
2019-08-13T20:08:44Z  
dc.date.issued
2018-11  
dc.identifier.citation
Acquaviva, Agustín; Jones, Andrew; Dennis, Gary R.; Castells, Cecilia Beatriz Marta; Shalliker, R. Andrew; et al.; Narrow bore post column derivatisation assays via reaction flow chromatography; Elsevier Science; Microchemical Journal; 142; 11-2018; 181-186  
dc.identifier.issn
0026-265X  
dc.identifier.uri
http://hdl.handle.net/11336/81579  
dc.description.abstract
This study highlights the capability to conduct a post column derivatisation (PCD) process with a narrow bore, low volume column. A reaction flow (RF) HPLC column (30 mm × 2.1 mm internal diameter (i.d.), 3 μm particle diameter) enabled the selective detection of phenols for both a simple standard mixture and a complex sample represented by green tea. The resolving power and sensitivity obtained were similar to an analytical scale (4.6 mm i.d.) RF HPLC column of the same format. However, the baseline noise experienced was higher when the assay was scaled down from the RF 4.6 mm i.d. to the RF 2.1 mm i.d. narrow bore column. The PCD mixing and reaction speed inside the RF column outlet (frit and head-fitting design) enabled the coupling of PCD protocols with “UHPLC-type” low volume columns and systems without significant loss in separation performance. In comparison to a conventional analytical scale PCD approach the scaled down narrow bore RF approach demonstrated 40 times greater sensitivity in terms of S/N. Scaled down PCD is vital for decreased consumption and cost without significant loss in separation performance.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Narrow Bore  
dc.subject
Phenolics  
dc.subject
Post Column Derivatisation  
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Reaction Flow Chromatography  
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Scaled Down  
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Selective Detection  
dc.subject.classification
Química Analítica  
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Ciencias Químicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Narrow bore post column derivatisation assays via reaction flow chromatography  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-08-08T16:12:20Z  
dc.journal.volume
142  
dc.journal.pagination
181-186  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Acquaviva, Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina  
dc.description.fil
Fil: Jones, Andrew. Western Sydney University; Australia  
dc.description.fil
Fil: Dennis, Gary R.. Western Sydney University; Australia  
dc.description.fil
Fil: Castells, Cecilia Beatriz Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Facultad de Ciencias Exactas, Universidad Nacional de la Plata; Argentina  
dc.description.fil
Fil: Shalliker, R. Andrew. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Western Sydney University; Australia  
dc.description.fil
Fil: Soliven, Arianne. Universidad de la República; Uruguay  
dc.journal.title
Microchemical Journal  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X18306957  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.microc.2018.06.032