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dc.contributor.author
Gholipour, Yousef
dc.contributor.author
Erra Balsells, Rosa
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dc.contributor.author
Hiraoka, Kenzo
dc.contributor.author
Nonami, Hiroshi
dc.date.available
2016-11-11T18:54:25Z
dc.date.issued
2013-01
dc.identifier.citation
Gholipour, Yousef; Erra Balsells, Rosa; Hiraoka, Kenzo; Nonami, Hiroshi; Living Cell Manipulation, Manageable Sampling, and Shotgun Picoliter Electrospray Mass Spectrometry for Profiling Metabolites; Elsevier; Analytical Biochemistry; 433; 1; 1-2013; 70-78
dc.identifier.issn
0003-2697
dc.identifier.uri
http://hdl.handle.net/11336/8153
dc.description.abstract
A modified cell pressure probe and an online Orbitrap mass spectrometer were used to sample in situ plant single cells without any additional manipulation. The cell pressure probe, a quartz capillary tip filled with an oil mixture, was penetrated to various depths into parenchyma cells of tulip bulb scale, followed by a hydraulic continuity test to determine the exact location of the tip inside target cells. The operation was conducted under a digital microscope, and the capillary tip was photographed to calculate the volume of the cell sap sucked. The cell sap sample was then directly nebulized/ionized under high-voltage conditions at the entrance of the mass spectrometer. Several sugars, amino acids, organic acids, vitamins, fatty acids, and secondary metabolites were detected. Because picoliter solutions can be accurately handled and measured, known volumes of standard solutions can be added to cell sap samples inside the capillary tip to be used as references for metabolite characterization and relative quantitation. The high precision and sensitivity of the cell pressure probe and Orbitrap mass spectrometer allow for the manipulation and analysis of both femtoliter cell sap samples and standard solutions.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Ms
dc.subject
Esi
dc.subject
Ppesi
dc.subject
Ppesi-Ms
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Living Cell Manipulation, Manageable Sampling, and Shotgun Picoliter Electrospray Mass Spectrometry for Profiling Metabolites
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-11-11T15:08:36Z
dc.journal.volume
433
dc.journal.number
1
dc.journal.pagination
70-78
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Gholipour, Yousef. Ehime University; Japón
dc.description.fil
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono; Argentina
dc.description.fil
Fil: Hiraoka, Kenzo. University of Yamanash; Japón
dc.description.fil
Fil: Nonami, Hiroshi. Ehime University; Japón
dc.journal.title
Analytical Biochemistry
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0003269712004952
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ab.2012.10.001
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