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dc.contributor.author
Griet, Milagros
dc.contributor.author
Font, Graciela Maria
dc.contributor.author
Gerez, Carla Luciana
dc.contributor.author
Rodriguez, Ana Virginia
dc.date.available
2019-08-12T17:09:11Z
dc.date.issued
2018-07
dc.identifier.citation
Griet, Milagros; Font, Graciela Maria; Gerez, Carla Luciana; Rodriguez, Ana Virginia; Two-step production of anti-inflammatory soluble factor by Lactobacillus reuteri CRL 1098; Public Library of Science; Plos One; 13; 7; 7-2018; 1-17
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/81438
dc.description.abstract
We have demonstrated previously that a soluble factor (LrS) produced by Lactobacillus (L.) reuteri CRL 1098 modulates the inflammatory response triggered by lipopolysaccharide. In this study, the production of LrS by L. reuteri CRL 1098 was realized through two steps: i)bacterial biomass production, ii) LrS production, where the bacterial biomass was able to live but did not proliferate. Therefore, the simultaneous evaluation of the effect of different factors on the growth and LrS production was performed. Biomass production was found to be dependent mainly on culture medium, while LrS production with anti-inflammatory activity depended on culture conditions of the biomass such as pH, agitation and growth phase. The L. reuteri CRL 1098 biomass and LrS production in the optimized culture media designed for this work reduced the complete process cost by approximately 95%, respectively to laboratory scale cost.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Two-Step Production
dc.subject
Anti-Inflammatory Soluble Factor
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Lactobacillus Reuteri
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Two-step production of anti-inflammatory soluble factor by Lactobacillus reuteri CRL 1098
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-07-24T18:02:27Z
dc.journal.volume
13
dc.journal.number
7
dc.journal.pagination
1-17
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco, CA
dc.description.fil
Fil: Griet, Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
dc.description.fil
Fil: Gerez, Carla Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Rodriguez, Ana Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://dx.plos.org/10.1371/journal.pone.0200426
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0200426
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