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dc.contributor.author
Marques, Viviane F.
dc.contributor.author
de Souza, Miliane M.S.
dc.contributor.author
de Mendonça, Elaine C.I.
dc.contributor.author
Abreu de Alencar, Tatiani
dc.contributor.author
Rocha Pribul, Bruno
dc.contributor.author
de O. Coelho, Shana de M.
dc.contributor.author
Lasagno, Mirta Cristina
dc.contributor.author
Reinoso, Elina Beatríz
dc.date.available
2015-06-18T16:52:10Z
dc.date.issued
2013-04
dc.identifier.citation
Marques, Viviane F.; de Souza, Miliane M.S.; de Mendonça, Elaine C.I.; Abreu de Alencar, Tatiani; Rocha Pribul, Bruno; et al.; Analise fenotípica e genotípica da virulência de Staphylococcus spp. e de sua dispersão clonal como contribuição ao estudo da mastite bovina; Revista Pesquisa Veterinaria Brasileira; Pesquisa Veterinária Brasileira; 33; 2; 4-2013; 161-170
dc.identifier.issn
0100-736X
dc.identifier.uri
http://hdl.handle.net/11336/812
dc.description.abstract
Mastitis is an inflammation of one or more mammary glands caused mainly by bacte- ria, among which the genus Staphylococcus plays an important role. Bacteria belonging to this genus are known to express virulence factors which allow their persistence and spread in the host. This study aimed to evaluate the phenotypic and genotypic aspects of virulence factors in Staphylococci spp. isolates from bovine mastitis clinical cases. A total of 272 milk samples from 8 farms in the South-Fluminense region of Rio de Janei- ro were analyzed. The samples underwent conventional bacterial identification, yielding 250 Staphylococci spp. isolates. These were tested for the phenotypic detection of slime production by the microplate and Congo Red Agar methods. The hemolysins production, hemolytic synergism, caseinase and DNase production were also evaluated. The isolates were then assayed through the Polymerase Chain Reaction method to detect genes asso- ciated with virulence factors such as: capsule (cap5, cap8), fibronectin (fnbA, fnbB), slime (icaA, icaD) and hemolysins (hla e hlb). Regarding the number of isolates assessed, 58% (145/250) were identified as coagulase-negative Staphylococcus spp. and 42% (105/250) as coagulase-positive Staphylococcus spp. The latter comprised 36.2% (38/105) of isolates identified as S. aureus, 11.4% (12/105) as S. intermedius and 3.8% (4/105) belonging to the SIG group. The hemolisin production was not significant, whereas only 6,4% (16/250) produced alfa hemolysis, 4,8% (12/250) produced beta hemolysis and 1,6% (4/250) was able to produce both. Caseinase production was observed in 66.4% (166/250) and sli- me production assayed through the microplate method was positive in 76,8% (192/250). DNAse was detected in coagulase-negative Staphylococcus spp. (38/145) and in S. aureus (14/38). Low association between genetic detection of icaA (38/250) and icaD (54/250) and slime phenotypic expression (192/250) suggest that others genetic markers can be involved in this expression. Regarding gene amplification, the isolates did not show sig- nificant correlation between the genetic detection of icaA (38/250) and icaD (54/250) and slime production (192/250), indicating that other genetic markers may be involved in this trait expression. The frequency of the occurrence of the others studied genes was of 4% (10/250) for cap5 and cap8, 32,8% (82/250) for fnbA, 4,4% (11/250) for fnbB, 19,2% (48/250) for hla and 18% (45/250) for hlb. The major circulating strain profile on the farms encompassed slime and caseinase producer strains. The spaA gene was found in all of the S. aureus isolates, presenting varying amplicons sizes, with 300bp being the prevalent size. The amplification of the coa gene showed nine polymorphic variants, with 600bp being the prevalent amplicon. The agr gene was also detected in every S. aureus isolate, with an amplicon of 200 bp. It was noticed that the presence or absence of the virulence genes in this study were not correlated with the 6 distinct electrophoretic profiles obtained by PFGE.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Revista Pesquisa Veterinaria Brasileira
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
S Aureus
dc.subject
Mastitis
dc.subject.classification
Ciencias Naturales y Exactas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
Biología Celular, Microbiología
dc.title
Analise fenotípica e genotípica da virulência de Staphylococcus spp. e de sua dispersão clonal como contribuição ao estudo da mastite bovina
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.identifier.eissn
1678-5150
dc.journal.volume
33
dc.journal.number
2
dc.journal.pagination
161-170
dc.journal.pais
Brasil
dc.description.fil
Fil: Marques, Viviane F.. Universidade Federal Rural do Rio de Janeiro; Brasil;
dc.description.fil
Fil: de Souza, Miliane M.S.. Universidade Federal Rural do Rio de Janeiro; Brasil;
dc.description.fil
Fil: de Mendonça, Elaine C.I.. Universidade Federal Rural do Rio de Janeiro; Brasil;
dc.description.fil
Fil: Abreu de Alencar, Tatiani. Universidade Federal Rural do Rio de Janeiro; Brasil;
dc.description.fil
Fil: Rocha Pribul, Bruno. Universidade Federal Rural do Rio de Janeiro; Brasil;
dc.description.fil
Fil: de O. Coelho, Shana de M.. Universidade Federal Rural do Rio de Janeiro; Brasil;
dc.description.fil
Fil: Lasagno, Mirta Cristina. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Area de Bacteriologia; Argentina;
dc.description.fil
Fil: Reinoso, Elina Beatríz. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia; Argentina;
dc.journal.title
Pesquisa Veterinária Brasileira
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/scielo.php?pid=S0100-736X2013000200005&script=sci_arttext


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