Mostrar el registro sencillo del ítem

dc.contributor.author
Galardo, Maria Noel Lujan
dc.contributor.author
Riera, Maria Fernanda
dc.contributor.author
Regueira, Mariana
dc.contributor.author
Pellizzari, Eliana Herminia
dc.contributor.author
Cigorraga, Selva Beatriz
dc.contributor.author
Meroni, Silvina Beatriz
dc.date.available
2016-11-10T17:58:40Z
dc.date.issued
2013-05
dc.identifier.citation
Galardo, Maria Noel Lujan; Riera, Maria Fernanda; Regueira, Mariana; Pellizzari, Eliana Herminia; Cigorraga, Selva Beatriz; et al.; Different signal transduction pathways elicited by basic fibroblast growth factor and interleukin 1β regulate CREB phosphorylation in Sertoli cells; Springer; Journal Of Endocrinological Investigation; 36; 5; 5-2013; 331-338
dc.identifier.issn
0391-4097
dc.identifier.uri
http://hdl.handle.net/11336/8126
dc.description.abstract
Background and aim: Basic fibroblast growth factor (bFGF) and interleukin 1β (IL1β) belong to the set of intratesticular regulators that provide for the fine-tuning of processes implicated in the maintenance of spermatogenesis. The aim of this study was to investigate if bFGF and IL1β activate CREB, what signaling pathways may be participating and the possible relationship between CREB activation and the regulation of Sertoli cell function. Methods: Twenty-day-old rat Sertoli cell cultures were used. Results: Cultures stimulated with bFGF and IL1β produced a time-dependent increment in phosphorylated CREB levels that reached maximal values in 5- and 15-minute incubations respectively. MEK inhibitors — PD98059 and U0126 — blocked the effect of bFGF on phosphorylated CREB while a p38-MAPK inhibitor — SB203580 — blocked the effect of IL1 β on phosphorylated CREB. A possible correlation between CREB regulation and two Sertoli cell-differentiated functions, Ldh A and transferrin expression, was explored. PD98059 blocked the ability of bFGF to stimulate Ldh A expression and SB203580 blocked the ability of IL1 β to stimulate Ldh A expression and LDH activity. Concerning transferrin, PD98059 and U0126 were able to inhibit the ability of bFGF to stimulate its secretion. On the contrary, SB203580 was unable to block IL1β induced increase in transferrin secretion suggesting that the p38-MAPK pathway does not participate in the mechanism of action of the cytokine to regulate transferrin. Conclusions: The results presented herein suggest that CREB is stimulated in response to bFGF and IL1 β through p42/p44-MAPK and p38-MAPK pathways and that this transcription factor may be partially responsible for the regulation of Sertoli cell function.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Testis
dc.subject
Sertoli
dc.subject
Paracrine Factors
dc.subject
Creb
dc.subject
Signal Transducion
dc.subject.classification
Otras Ciencias Médicas
dc.subject.classification
Otras Ciencias Médicas
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Different signal transduction pathways elicited by basic fibroblast growth factor and interleukin 1β regulate CREB phosphorylation in Sertoli cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-11-10T13:49:01Z
dc.journal.volume
36
dc.journal.number
5
dc.journal.pagination
331-338
dc.journal.pais
Italia
dc.description.fil
Fil: Galardo, Maria Noel Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.description.fil
Fil: Riera, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.description.fil
Fil: Regueira, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.description.fil
Fil: Pellizzari, Eliana Herminia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.description.fil
Fil: Cigorraga, Selva Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.description.fil
Fil: Meroni, Silvina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
dc.journal.title
Journal Of Endocrinological Investigation
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.3275/8582
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3275/8582


Archivos asociados

Documento no disponible

Comunidades y colecciones

  • Articulos(CEDIE) [112]
    Articulos de CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"

Mostrar el registro sencillo del ítem