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dc.contributor.author
Gamarra Luques, Carlos Diego  
dc.contributor.author
Goyeneche, Alicia A.  
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Hapon, María Belén  
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Telleria, Carlos Marcelo  
dc.date.available
2019-08-07T20:27:51Z  
dc.date.issued
2012-05  
dc.identifier.citation
Gamarra Luques, Carlos Diego; Goyeneche, Alicia A.; Hapon, María Belén; Telleria, Carlos Marcelo; Mifepristone prevents repopulation of ovarian cancer cells escaping cisplatin-paclitaxel therapy; BioMed Central; BMC Cancer; 12; 5-2012; 1-16  
dc.identifier.issn
1471-2407  
dc.identifier.uri
http://hdl.handle.net/11336/81171  
dc.description.abstract
Background: Advanced ovarian cancer is treated with cytoreductive surgery and combination platinum- and taxane-based chemotherapy. Although most patients have acute clinical response to this strategy, the disease ultimately recurs. In this work we questioned whether the synthetic steroid mifepristone, which as monotherapy inhibits the growth of ovarian cancer cells, is capable of preventing repopulation of ovarian cancer cells if given after a round of lethal cisplatin-paclitaxel combination treatment.Methods: We established an in vitro approach wherein ovarian cancer cells with various sensitivities to cisplatin or paclitaxel were exposed to a round of lethal doses of cisplatin for 1 h plus paclitaxel for 3 h. Thereafter, cells were maintained in media with or without mifepristone, and short- and long-term cytotoxicity was assessed.Results: Four days after treatment the lethality of cisplatin-paclitaxel was evidenced by reduced number of cells, increased hypodiploid DNA content, morphological features of apoptosis, DNA fragmentation, and cleavage of caspase-3, and of its downstream substrate PARP. Short-term presence of mifepristone either enhanced or did not modify such acute lethality. Seven days after receiving cisplatin-paclitaxel, cultures showed signs of relapse with escaping colonies that repopulated the plate in a time-dependent manner. Conversely, cultures exposed to cisplatin-paclitaxel followed by mifepristone not only did not display signs of repopulation following initial chemotherapy, but they also had their clonogenic capacity drastically reduced when compared to cells repopulating after cisplatin-paclitaxel.Conclusions: Cytostatic concentrations of mifepristone after exposure to lethal doses of cisplatin and paclitaxel in combination blocks repopulation of remnant cells surviving and escaping the cytotoxic drugs.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
BioMed Central  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Mifepristone  
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Cancer de Ovario  
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Apoptosis  
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Ciclo Celular  
dc.subject.classification
Inmunología  
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Medicina Básica  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Mifepristone prevents repopulation of ovarian cancer cells escaping cisplatin-paclitaxel therapy  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-06-10T21:20:37Z  
dc.journal.volume
12  
dc.journal.pagination
1-16  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Londres  
dc.description.fil
Fil: Gamarra Luques, Carlos Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. University Of South Dakota. Sanford School Of Medicine; Estados Unidos  
dc.description.fil
Fil: Goyeneche, Alicia A.. University Of South Dakota. Sanford School Of Medicine; Estados Unidos  
dc.description.fil
Fil: Hapon, María Belén. University Of South Dakota. Sanford School Of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina  
dc.description.fil
Fil: Telleria, Carlos Marcelo. University Of South Dakota. Sanford School Of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.journal.title
BMC Cancer  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-12-200  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/1471-2407-12-200