Mostrar el registro sencillo del ítem
dc.contributor.author
Belov, Vladimir N.
dc.contributor.author
Bossi, Mariano Luis
dc.date.available
2016-11-09T21:25:49Z
dc.date.issued
2013-05
dc.identifier.citation
Belov, Vladimir N. ; Bossi, Mariano Luis; Photoswitching Emission with Rhodamine Spiroamides for Superresolution Fluorescence nanoscopies; Wiley; Israel Journal of Chemistry; 53; 5; 5-2013; 267-279
dc.identifier.issn
1869-5868
dc.identifier.uri
http://hdl.handle.net/11336/8114
dc.description.abstract
Spatial resolution in far-field fluorescence microscopy is limited by diffraction to about 200 nm. With the aid of photoswitchable fluorophores, the diffraction barrier has been successfully overcome, allowing unprecedented resolution in the order of single biomolecules. The imaging process demands markers with strict and reliable control of the switching, to keep most of the markers in a non-emissive state most of the time and to bring a tiny number back to an emissive state, and detection at the single-molecule level. Herein, we describe the use of rhodamine spiroamides with unique photophysical properties as molecular probes for super-resolution techniques based on the localization of single emitters. This family of photochromic and fluorescent compounds fulfils the stringent requirements for such imaging methods; these compounds are robust and capable of enduring single-molecule detection in diverse environments. This has allowed meaningful images with resolution down to a few nanometres. Their design, synthesis and implementation is discussed along with imaging applications in material and life sciences.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Fluorescence
dc.subject
Optical Nanoscopy
dc.subject
Photochemistry
dc.subject
Photochromism
dc.subject.classification
Físico-Química, Ciencia de los Polímeros, Electroquímica
dc.subject.classification
Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Photoswitching Emission with Rhodamine Spiroamides for Superresolution Fluorescence nanoscopies
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-10-25T19:27:28Z
dc.journal.volume
53
dc.journal.number
5
dc.journal.pagination
267-279
dc.journal.pais
Alemania
dc.journal.ciudad
Weinheim
dc.description.fil
Fil: Belov, Vladimir N. . The Max Planck Institute For Biophysical Chemistry; Alemania
dc.description.fil
Fil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; Argentina
dc.journal.title
Israel Journal of Chemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/ijch.201300017/abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/ijch.201300017
Archivos asociados