Repositorio Institucional
Repositorio Institucional
CONICET Digital
  • Inicio
  • EXPLORAR
    • AUTORES
    • DISCIPLINAS
    • COMUNIDADES
  • Estadísticas
  • Novedades
    • Noticias
    • Boletines
  • Ayuda
    • General
    • Datos de investigación
  • Acerca de
    • CONICET Digital
    • Equipo
    • Red Federal
  • Contacto
JavaScript is disabled for your browser. Some features of this site may not work without it.
  • INFORMACIÓN GENERAL
  • RESUMEN
  • ESTADISTICAS
 
Artículo

New tools to screen wild peanut species for aflatoxin accumulation and genetic fingerprinting

Arias, Renee S.; Sobolev, Victor S.; Massa, Alicia N.; Orner, Valerie A.; Walk, Travis E.; Ballard, Linda L.; Simpson, Sheron A.; Puppala, Naveen; Scheffler, Brian E.; de Blas, Francisco JavierIcon ; Seijo, José GuillermoIcon
Fecha de publicación: 08/2018
Editorial: BioMed Central
Revista: BMC Plant Biology
e-ISSN: 1471-2229
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Nutrición, Dietética

Resumen

Aflatoxin contamination in peanut seeds is still a serious problem for the industry and human health. No stable aflatoxin resistant cultivars have yet been produced, and given the narrow genetic background of cultivated peanuts, wild species became an important source of genetic diversity. Wild peanut seeds, however, are not abundant, thus, an effective method of screening for aflatoxin accumulation using minimal seeds is highly desirable. In addition, keeping record of genetic fingerprinting of each accession would be very useful for breeding programs and for the identification of accessions within germplasm collections. Results: In this study, we report a method of screening for aflatoxin accumulation that is applicable to the small-size seeds of wild peanuts, increases the reliability by testing seed viability, and records the genetic fingerprinting of the samples. Aflatoxin levels observed among 20 wild peanut species varied from zero to 19000 ng.g-1 and 155 ng.g-1 of aflatoxin B1 and B2, respectively. We report the screening of 373 molecular markers, including 288 novel SSRs, tested on 20 wild peanut species. Multivariate analysis by Neighbor-Joining, Principal Component Analysis and 3D-Principal Coordinate Analysis using 134 (36 %) transferable markers, in general grouped the samples according to their reported genomes. The best 88 markers, those with high fluorescence, good scorability and transferability, are reported with BLAST results. High quality markers (total 98) that discriminated genomes are reported. A high quality marker with UPIC score 16 (16 out of 20 species discriminated) had significant hits on BLAST2GO to a pentatricopeptide-repeat protein, another marker with score 5 had hits on UDP-D-apiose synthase, and a third one with score 12 had BLASTn hits on La-RP 1B protein. Together, these three markers discriminated all 20 species tested. Conclusions: This study provides a reliable method to screen wild species of peanut for aflatoxin resistance using minimal seeds. In addition we report 288 new SSRs for peanut, and a cost-effective combination of markers sufficient to discriminate all 20 species tested. These tools can be used for the systematic search of aflatoxin resistant germplasm keeping record of the genetic fingerprinting of the accessions tested for breeding purpose.
Palabras clave: Aflatoxin , Arachis , Aspergillus Flavus , Fingerprinting , Groundnut , Molecular Markers , Peanut
Ver el registro completo
 
Archivos asociados
Thumbnail
 
Tamaño: 1.660Mb
Formato: PDF
.
Descargar
Licencia
info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/80794
URL: https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-018-1355-9
DOI: https://dx.doi.org/10.1186%2Fs12870-018-1355-9
Colecciones
Articulos(IBONE) [807]
Articulos de INST.DE BOTANICA DEL NORDESTE (I)
Articulos(IMBIV) [2023]
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA VEGETAL (P)
Citación
Arias, Renee S.; Sobolev, Victor S.; Massa, Alicia N.; Orner, Valerie A.; Walk, Travis E.; et al.; New tools to screen wild peanut species for aflatoxin accumulation and genetic fingerprinting; BioMed Central; BMC Plant Biology; 18; 1; 8-2018; 1-13
Compartir
Altmétricas
 
Estadísticas
Visualizaciones: 92
Descargas: 160

Enviar por e-mail
Separar cada destinatario (hasta 5) con punto y coma.
  • Facebook
  • Twitter
  • Instagram
  • YouTube
  • Sound Cloud

Los contenidos del CONICET están licenciados bajo Creative Commons Reconocimiento 2.5 Argentina License

Ministerio
https://www.conicet.gov.ar/ - CONICET

Inicio

Explorar

  • Autores
  • Disciplinas
  • Comunidades

Estadísticas

Novedades

  • Noticias
  • Boletines

Ayuda

Acerca de

  • CONICET Digital
  • Equipo
  • Red Federal

Contacto

Godoy Cruz 2290 (C1425FQB) CABA – República Argentina – Tel: +5411 4899-5400 repositorio@conicet.gov.ar
TÉRMINOS Y CONDICIONES