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Artículo

Wingless-type family member 3A (Wnt3a) triggers polarization of hippocampal neurons via cross-activation of the IGF-1 receptor/PI3k pathway

Bernis, Maria EugeniaIcon ; Oksdath, Mariana; Dupraz, Sebastian, EnriqueIcon ; Nieto Guil, Alvaro FernandoIcon ; Fernández, Marisa MarielIcon ; Malchiodi, Emilio LuisIcon ; Rosso, Silvana BeatrizIcon ; Quiroga, SantiagoIcon
Fecha de publicación: 10/2013
Editorial: Frontiers Research Foundation
Revista: Front Cell Neurosci
e-ISSN: 1662-5102
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Initial axonal elongation is essential for neuronal polarization and requires polarized activation of IGF-1 receptors (IGF-1r) and the phosphatidylinositol 3 kinase (PI3k) pathway. Wingless-type family growth factors (Wnts) have also been implied in the regulation of axonal development. It is not known, however, if Wnts have any participation in the regulation of initial axonal outgrowth and the establishment of neuronal polarity. We used cultured hippocampal neurons and growth cone particles (GCPs) isolated from fetal rat brain to show that stimulation with the wingless family factor 3A (Wnt3a) was sufficient to promote neuronal polarization in the absence of IGF-1 or high insulin. We also show that Wnt3a triggered a strong activation of IGF-1r, PI3k, and Akt in developmental Stage 2 neurons and that the presence of activatable IGF-1r and PI3k activation were necessary for Wnt3a polarizing effects. Surface plasmon resonance (SPR) experiments show that Wnt3a did not bind specifically to the IGF-1r. Using crosslinking and immuno-precipitation experiments, we show that stimulation with Wnt3a triggered the formation of a complex including IGF-1r-Wnt3a-Frizzled-7. We conclude that Wnt3a triggers polarization of neurons via cross-activation of the IGF-1r/PI3k pathway upon binding to Fz7.
Palabras clave: Wnt3a , Igf-1 Receptor
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution 2.5 Unported (CC BY 2.5)
Identificadores
URI: http://hdl.handle.net/11336/8066
URL: http://journal.frontiersin.org/article/10.3389/fncel.2013.00194/full
DOI: http://dx.doi.org/10.3389/fncel.2013.00194
URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829478/
Colecciones
Articulos(IDEHU)
Articulos de INST.DE EST.DE LA INMUNIDAD HUMORAL PROF.R.A.MARGNI
Citación
Bernis, Maria Eugenia; Oksdath, Mariana; Dupraz, Sebastian, Enrique; Nieto Guil, Alvaro Fernando; Fernández, Marisa Mariel; et al.; Wingless-type family member 3A (Wnt3a) triggers polarization of hippocampal neurons via cross-activation of the IGF-1 receptor/PI3k pathway; Frontiers Research Foundation; Front Cell Neurosci; 7; 194; 10-2013; 1-12
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