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dc.contributor.author
Ferrero, Mariana Cristina
dc.contributor.author
Hielpos, María Soledad
dc.contributor.author
Carvalho, Natalia
dc.contributor.author
Barrionuevo, Paula
dc.contributor.author
Corsetti, Patricia
dc.contributor.author
Giambartolomei, Guillermo Hernan
dc.contributor.author
Costa Oliveira, Sergio
dc.contributor.author
Baldi, Pablo Cesar
dc.date.available
2016-11-07T20:13:39Z
dc.date.issued
2014-02
dc.identifier.citation
Ferrero, Mariana Cristina; Hielpos, María Soledad; Carvalho, Natalia; Barrionuevo, Paula; Corsetti, Patricia; et al.; Key role of TLR2 in the inflammatory response and MHC-II down-regulation in Brucella-infected alveolar macrophages; American Society For Microbiology; Infection And Immunity; 82; 2; 2-2014; 626-639
dc.identifier.issn
0019-9567
dc.identifier.uri
http://hdl.handle.net/11336/8035
dc.description.abstract
Alveolar macrophages (AM) seem to constitute the main cellular target of inhaled brucellae. Here we show that Brucella abortus invades and replicates in murine AM without inducing cytotoxicity. B. abortus infection induced a statistically significant increase of TNF-α, KC, IL-1β, IL-6 and IL-12 in AM from C57BL/6 mice and Balb/c mice, but these responses were generally weaker and/or delayed as compared to those elicited in peritoneal macrophages. Studies using knockout mice for TLR2, TLR4 and TLR9 revealed that TNF-α and KC responses were mediated by TLR2 recognition. Brucella infection reduced in a MOI-dependent manner the expression of MHC-II molecules induced by gamma-interferon (IFN-γ) in AM. The same phenomenon was induced by incubation with heat-killed B. abortus (HKBA) or the lipidated form of the 19 kDa outer membrane protein of Brucella (L-Omp19), and was shown to be mediated by TLR2 recognition. In contrast, no significant down-regulation of MHC-II was induced by either unlipidated Omp19 or Brucella LPS. In a functional assay, treatment of AM with either L-Omp19 or HKBA reduced the MHC-II-restricted presentation of OVA peptides to specific T cells. One week after intra-tracheal infection viable B. abortus was detected in AM from both wild type and TLR2 KO mice, but CFU counts were higher in the latter. These results suggest that B. abortus may survive in AM after inhalatory infection in spite of a certain degree of immune control exerted by the TLR2-mediated inflammatory response. Both the modest nature of the latter and the modulation of MHC-II expression by the bacterium may contribute to such survival.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society For Microbiology
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Brucella
dc.subject
Alveolar Macrophages
dc.subject
Innate Immunity
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Key role of TLR2 in the inflammatory response and MHC-II down-regulation in Brucella-infected alveolar macrophages
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-11-07T15:42:36Z
dc.journal.volume
82
dc.journal.number
2
dc.journal.pagination
626-639
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: Ferrero, Mariana Cristina. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
dc.description.fil
Fil: Hielpos, María Soledad. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
dc.description.fil
Fil: Carvalho, Natalia. Universidade Federal Do Minas Gerais. Instituto de Cs.biologicas; Brasil
dc.description.fil
Fil: Barrionuevo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; Argentina
dc.description.fil
Fil: Corsetti, Patricia. Universidade Federal Do Minas Gerais. Instituto de Cs.biologicas; Brasil
dc.description.fil
Fil: Giambartolomei, Guillermo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; Argentina
dc.description.fil
Fil: Costa Oliveira, Sergio. Universidade Federal Do Minas Gerais. Instituto de Cs.biologicas; Brasil
dc.description.fil
Fil: Baldi, Pablo Cesar. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
dc.journal.title
Infection And Immunity
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://iai.asm.org/content/82/2/626.full
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/IAI.01237-13


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