Repositorio Institucional
Repositorio Institucional
CONICET Digital
  • Inicio
  • EXPLORAR
    • AUTORES
    • DISCIPLINAS
    • COMUNIDADES
  • Estadísticas
  • Novedades
    • Noticias
    • Boletines
  • Ayuda
    • General
    • Datos de investigación
  • Acerca de
    • CONICET Digital
    • Equipo
    • Red Federal
  • Contacto
JavaScript is disabled for your browser. Some features of this site may not work without it.
  • INFORMACIÓN GENERAL
  • RESUMEN
  • ESTADISTICAS
 
Artículo

Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral Chagas disease in a patient with AIDS

Burgos, Juan MiguelIcon ; Bergher, Sandra B.; Freitas, Jorge M.; Bisio, Margarita María CatalinaIcon ; Altcheh, Jaime MarceloIcon ; Teijeiro, Ricardo; Begher, Sandra B.; Freilij, Hector León; Deccarlini, Florencia; Levalle, Jorge; Lopez Alcoba, Horacio; Burgos, Juan MiguelIcon ; Levin, Mariano JorgeIcon ; Duffy, TomásIcon ; Macedo, Andrea M.; Schijman, Alejandro GabrielIcon
Fecha de publicación: 12/2005
Editorial: American Society of Tropical Medicine and Hygiene
Revista: American Journal of Tropical Medicine and Hygiene
ISSN: 0002-9637
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Medicina Tropical

Resumen

Trypanosoma cruzi DNA was amplified from an intracranial biopsy and peripheral blood of an HIV patient with encephalitis; this episode was indicative of AIDS and congenital Chagas disease. The analysis of a microsatellite locus revealed a multiclonal parasite population at the brain lesion with a more complex minicircle signature than that profiled in blood using restriction fragment length polymorphism (RFLP)-PCR and low stringency single primer (LSSP) PCR. Interestingly, different sublineages of T. cruzi II were detected in blood and brain by means of spliced-leader and 24s ribosomal-DNA amplifications. Quantitative-competitive PCR monitored the decrease of parasitic load during treatment and secondary prophylaxis with benznidazole. The synergy between parasiticidal plus antiretroviral treatments probably allowed the patient a longer survival than usually achieved in similar episodes. This is the first case report demonstrating a differential distribution of natural parasite populations and sublineages in Chagas disease reactivation, showing the proliferation of cerebral variants not detectable in peripheral blood.
Palabras clave: Trypanosoma Crusi , Chagas , Aids
Ver el registro completo
 
Archivos asociados
Thumbnail
 
Tamaño: 125.8Kb
Formato: PDF
.
Descargar
Licencia
info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/80101
DOI: https://doi.org/10.4269/ajtmh.2005.73.1016
URL: http://www.ajtmh.org/content/journals/10.4269/ajtmh.2005.73.1016
Colecciones
Articulos(INGEBI)
Articulos de INST.DE INVEST.EN ING.GENETICA Y BIOL.MOLECULAR "DR. HECTOR N TORRES"
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Burgos, Juan Miguel; Bergher, Sandra B.; Freitas, Jorge M.; Bisio, Margarita María Catalina; Altcheh, Jaime Marcelo; et al.; Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral Chagas disease in a patient with AIDS; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 73; 6; 12-2005; 1016-1018
Compartir
Altmétricas
 

Enviar por e-mail
Separar cada destinatario (hasta 5) con punto y coma.
  • Facebook
  • X Conicet Digital
  • Instagram
  • YouTube
  • Sound Cloud
  • LinkedIn

Los contenidos del CONICET están licenciados bajo Creative Commons Reconocimiento 2.5 Argentina License

https://www.conicet.gov.ar/ - CONICET

Inicio

Explorar

  • Autores
  • Disciplinas
  • Comunidades

Estadísticas

Novedades

  • Noticias
  • Boletines

Ayuda

Acerca de

  • CONICET Digital
  • Equipo
  • Red Federal

Contacto

Godoy Cruz 2290 (C1425FQB) CABA – República Argentina – Tel: +5411 4899-5400 repositorio@conicet.gov.ar
TÉRMINOS Y CONDICIONES